Anti-seepage gel-electrophoresis precast gel shell

Abstract

The invention relates to an anti-seepage gel-electrophoresis precast gel shell. Horizontal connection edges are respectively arranged parallel to two sides of the bottom of the precast gel shell. Pasting areas of sealing membranes at two sides of the shell bottom are greatly increased, and the leakage problem of a gel premix liquid during the electrophoresis gel-making process is effectively avoided. The invention can be widely applied to precast gel shells of polyacrylamide gel electrophoresis, non-denaturing gel gradient electrophoresis, sodium dodecanesulphonate-polyacrylamide gel electrophoresis, two-dimensional gel electrophoresis, denaturing gradient polyacrylamide gel electrophoresis and single-cell gel electrophoresis, etc.

Description

technical field [0001] The invention relates to a casing of an anti-leakage gel electrophoresis prefabricated gel. Background technique [0002] Polyacrylamide gel electrophoresis (PAGE) is widely used in the separation and analysis of important biological substances such as proteins and nucleic acids. Currently, commonly used PAGE techniques include non-denaturing polyacrylamide gel electrophoresis (Native-PAGE, Schager, H., von Jagow, G., 1991, Anal. Biochem., 199, 223-231.), dodecylsulfonic acid Sodium-polyacrylamide gel electrophoresis (sodium dodecyl sulfate-polyacrylamide gel electrophoresis, SDS-PAGE, Weber, K., 1969, J. Biol. Chem. 244, 4406-4412.), two-dimensional electrophoresis (two dimensional electrophoresis , 2DE, Gorg, A., Postel, W., Gunther, S., Weser, J., 1985, Electrophoresis, 6, 599-604.), denatured gradient gel electrophoresis (denatured gradient gel electrophoresis, DGGE, Fischer, S.G., Lerman, L.S. , 1983, Proc.Natl.Acad.Sci., 80 (6), 1579-1583.) and...

AI Technical Summary

Problems solved by technology

The prefabricated glue production system relies on the glue making frame to make glue, but this pressure or fit-in fixed prefabricated glue shell method still uses the contact mode between the bottom of the prefabricated glue shell and the rubber pad to seal, and the size of the pressure and fit has a It is very subjective, and the repeated use of the gel pad will reduce its elasticity and flatness, resulting in the leakage of the premix solution during gel electrophoresis

In addition, when carrying out large-scale PAGE experiments, when multiple PAGE gels need to be gelled at the same time, the combination of the prefabricated gel shell and the gel frame seems to be insufficient.

The above problems not only greatly increase the workload of researchers, but also consume a lot of time.

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