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Collagen/chitosan composite sponge biological dressing and preparation method thereof

A technology of biological dressings and composite sponges, applied in medical science, absorbent pads, bandages, etc., can solve problems such as unsatisfactory antibacterial effects, achieve strong mechanical properties and stability, improve mechanical properties, and avoid structural collapse.

Active Publication Date: 2015-11-11
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There have been many reports about collagen and chitosan blending and spongy dressings prepared by freeze-drying. Biological tests have shown that such wound dressings have excellent repairing functions, but their antibacterial effects are not ideal.

Method used

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  • Collagen/chitosan composite sponge biological dressing and preparation method thereof
  • Collagen/chitosan composite sponge biological dressing and preparation method thereof
  • Collagen/chitosan composite sponge biological dressing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Nanometer TiO 2 Preparation and property analysis of hydrosol

[0037] 1.1nm TiO 2 Preparation method of hydrosol: Aiming at the requirement of collagen / chitosan composite sponge biological dressing, the present invention optimizes the scheme of preparing nano-titanium dioxide hydrosol by sol-gel technology through a large number of tests. For the convenience of analysis and comparison, the preparation of the nano-titanium dioxide hydrosol mentioned in the following examples is all prepared by the following method: take a certain molar ratio of tetrabutyl titanate, water, ethanol and concentrated hydrochloric acid (0.5~2:100~300 :10~20:0.5~1); first, mix water and concentrated hydrochloric acid uniformly in a single-necked flask, and stir with high-speed magnetic force at 45°C; then mix tetrabutyl titanate and ethanol uniformly, adjust pH=2 with concentrated hydrochloric acid, and The constant-pressure dropping funnel was evenly added dropwise in...

Embodiment 2

[0042] The impact of embodiment 2 nano-titanium dioxide addition on the performance of sponge biological dressing

[0043] 2.1 Preparation of sponge biological dressings with different nano-TiO content

[0044] Its preparation method is:

[0045] 1) Dissolve collagen and chitosan in 0.5M acetic acid solution to obtain 5g / L collagen solution and 5g / L chitosan solution;

[0046] 2) Mix chitosan solution and collagen solution according to the chitosan and collagen mass ratio of 1:1 to obtain collagen / chitosan mixture;

[0047] 3) The nano-titanium dioxide hydrosol was prepared by sol-gel technology, and the specific steps were the same as 1.1 in Example 1.

[0048] 4) According to the ratio of nano-titanium dioxide to the total weight of collagen and chitosan of 1%, 3%, 5%, and 7%, add nano-titanium dioxide hydrosol to the collagen / chitosan mixture, and vacuum defoam, After standing at 4°C for 12h, freeze-dry at -90~-30°C for 12h to obtain sample 1 (CCS-1%), sample 2 (CCS-...

Embodiment 3

[0107]A collagen / chitosan composite sponge biological dressing, which also includes nano-titanium dioxide, and nano-titanium dioxide accounts for 5% of the total weight of collagen and chitosan; the weight percentage of collagen and chitosan is: collagen 70%, chitosan Sugar 30%. The molecular weight of collagen is 200-300KDa. Collagen is extracted from pigskin by an acid-enzyme combination method. The degree of deacetylation of chitosan is 90%. The average particle size of the nano-titanium dioxide is 20-30nm.

[0108] The preparation method of collagen / chitosan composite sponge biological dressing comprises the following steps:

[0109] 1) Dissolve collagen and chitosan in 0.5M acetic acid solution to obtain 5g / L collagen solution and 5g / L chitosan solution;

[0110] 2) Mix the chitosan solution and the collagen solution according to the mass ratio of collagen and chitosan of 7:3 to obtain a collagen / chitosan mixture;

[0111] 3) Using sol-gel technology to prepare nano-...

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Abstract

The invention provides a collagen / chitosan composite sponge biological dressing, and belongs to the field of medical polymer materials. The biological dressing is compounded by collagen, chitosan and nano-titanium dioxide; the nano-titanium dioxide accounts for 1-5% of the total weight of the collagen and the chitosan; and weight percentage of the collagen is 50-70%, and the weight percentage of the chitosan is 30-50%. The wound dressing has good mechanical property; toxin residues caused by utilization of cross-linking agents and structure collapse caused by secondary freeze-drying are avoided; the problem of bacterial infection in a wound healing process can be solved effectively; and a sterile environment can be provided in a wound healing process.

Description

technical field [0001] The invention belongs to the field of medical polymer materials, and in particular relates to a collagen / chitosan composite sponge biological dressing and a preparation method thereof. Background technique [0002] Collagen is the main structural component of the extracellular matrix, which has excellent properties such as excellent biocompatibility, low antigenicity, high degradability, good hemostatic performance and promotion of cell growth and migration. Chitosan is a linear polysaccharide, which is the deacetylation product of chitin, and has excellent properties such as antibacterial properties, blood absorption capacity, biodegradability, and biocompatibility. The excellent properties of collagen and chitosan make them have broad application prospects in tissue engineering materials. [0003] There are a large number of hydrophilic groups on the surface of collagen and chitosan molecules, so that the composite sponge prepared by it has strong w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/28A61L15/46A61L15/44
Inventor 汤克勇范夏莲陈可可刘捷郑学晶裴莹王芳
Owner ZHENGZHOU UNIV
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