A kit, optical detection system and detection method for substance detection

Abstract

The invention provides a reagent box, an optical detection system and a detection method for substance detection. The reagent box has a simple structure, and the reagent can be conveniently fixed in the disc-shaped reagent box, which not only simplifies the operation required for the user to detect steps, it can also be applied to the detection of different substances in various situations; the optical detection system adopts a small rotating kit, and only one variable of the rotation angle needs to be controlled during detection, which simplifies the complexity of the system and increases the stability of the system ;A light source with three color channels that can be independently adjusted is used, so that the system can detect different substances through different parameter settings and feedback adjustments to the three color light sources; two different detection methods are used in different samples. When detecting the concentration of similar substances, the reliability of the detection results is enhanced through the calculation of the detection accuracy.

Description

technical field [0001] The invention relates to the detection field, in particular to a reagent box, an optical detection system and a detection method for substance detection. Background technique [0002] In recent years, optical detection of substances has been widely used in biology, chemical industry, quality inspection and other industries. Traditional optical detection methods can generally be divided into absorption detection and emission detection. Emission detection uses the fluorescence and other properties of a certain type of substance to measure its concentration by measuring the light emitted by the sample. Absorption detection, that is, to separate a certain monochromatic light and irradiate an unknown sample, and to detect the absorption rate of the sample to the light so as to know the concentration of the sample. [0003] The limitations of the above detection methods and detection systems are: [0004] 1. Ordinary samples have absorption behavior for l...

AI Technical Summary

Problems solved by technology

[0004] 1. Ordinary samples have absorption behavior for light of various wavelengths. Using narrow-wavelength light to measure the optical properties of a narrow bandwidth of the sample reduces the sensitivity of detection. Therefore, it is necessary to use extremely sensitive sensors, which increases the cost of detection.

[0005] 2. The current detection equipment is oriented to attributes rather than substances, so that users still need to carry out analysis brought into the standard curve to determine the concentration of substances after the detection is completed.

[0006] 3. Traditional low-cost small-scale optical detection equipment has a low degree of automation, which increases the difficulty of operation for users

However, high-cost testing equipment is bulky, which makes it difficult for users to detect on-site

[0007] 4. Traditional kits are mostly in the form of bottled reagents + square detection plates. This kit is suitable for the detection methods of large-scale equipment such as microplate readers, and is not suitable for miniaturized detection devices.

[0008] 5. The traditional method of outputting data from testing equipment to other equipment is mostly in the form of wired output such as computer serial port output or USB output. This output interface is suitable for analysis in fixed places, but it cannot realize user-oriented on-site testing.

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