Application of Turrapubin D in preparation of liver-protecting drug

A technology of turrapubind, 1. turrapubind is applied in the application field of Turrapubin D in the preparation of hepatoprotective drugs, and can solve problems such as no reports of compound activity yet

Inactive Publication Date: 2015-11-18
林天样
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is no report on the activity of this compound

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Turrapubin D in preparation of liver-protecting drug
  • Application of Turrapubin D in preparation of liver-protecting drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: Isolation, preparation and structure confirmation of TurrapubinD

[0010] The preparation method of TurrapubinD is the same as that reported in the literature (Bioactive Limonoid and Triterpenoid Constituents of Turraea pubescens, J. Nat. Prod., 2013, 76, 1166-1174).

[0011] Structural confirmation: According to HRESI-MS, the molecular formula is C 29 h 36 o 8 , with an unsaturation of 12. H NMR spectrum data δ H (ppm, DMSO-d 6 , 500MHz): H-1 (4.12, t, J=5.7), H-2 (2.85, dd, J=14.9, 5.7), H-2 (2.70, dd, J=14.9, 5.7), H-5 (2.63, dd, J=7.5, 3.6), H-6 (2.49, m), H-9 (3.38, d, J=8.7), H-11 (4.35, t, J=8.7), H-12 (5.47, d, J=8.7), H-15 (3.87, s), H-16 (1.80, m), H-16 (2.24, dd, J=14.0, 7.4), H-17 (2.99, dd , J=10.8, 7.4), H-18 (0.80, s), H-19 (1.06, s), H-21 (7.12, s), H-22 (6.16, s), H-23 (7.32, s), H-28 (0.96, s), H-29 (1.11, s), H-30 (5.30, s), 7-OMe (3.71, s), 12-OAc (1.89, s); NMR Carbon spectrum data δ C (ppm, DMSO-d 6 , 125Hz): 82.6 (CH, 1-C), 4...

Embodiment 2

[0013] Embodiment 2: Pharmacological action test of Turrapubin D

[0014] 1. Materials and Instruments

[0015] HL7702 liver cell line was provided by Shanghai Institute of Biological Sciences, Chinese Academy of Sciences. TurrapubinD is self-made, and the HPLC normalized purity is greater than 98%. High-glucose DMEM medium, high-glucose DMEM / F12=1:1 medium, and fetal bovine serum were all purchased from HycLone. EDTA was purchased from Shanghai Reagent No. 1 Factory. Trypan Blue was purchased from Beijing Chemical Plant. Sugar-free DMEM medium was purchased from Gibco. Trypsin, MTT, and DMSO were purchased from Amresco. LDH release assay kit was purchased from GENMDE. ALT biochemical detection kits, AST biochemical detection kits, and LDH biochemical detection kits were purchased from Beckman Reagent Co., Ltd., USA. Western and IP cell lysates, PMSF, BCA protein concentration determination kits, and MDA detection kits were all purchased from Beyontien Biotechnology Res...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
degree of unsaturationaaaaaaaaaa
Login to view more

Abstract

The invention discloses an application of Turrapubin D in preparation of a liver-protecting drug and belongs to the field of medicines. In vitro tests prove that Turrapubin D can enhance tolerance of liver cells to ischemia reperfusion injury in future, and expressions comprise that activity of the liver cells is enhanced and cell death rate is reduced, so that Turrapubin D pre-treatment can alleviate the ischemia reperfusion injury of human liver cells, and Turrapubin D can protect the liver cells and further can be prepared into a liver-protecting drug through research and development.

Description

technical field [0001] The invention relates to a new application of compound TurrapubinD, in particular to the application of TurrapubinD in the preparation of liver-protecting drugs. Background technique [0002] Chun-MaoYuan et al. isolated and purified the compound TurrapubinD for the first time, and published the results in the famous Journal of Natural Products (Bioactive Limonoid and Triterpenoid Constituents of Turraeapubescens, J.Nat.Prod., 2013, 76, 1166-1174). [0003] There is no activity report of this compound yet. Contents of the invention [0004] The object of the present invention is to provide a medical application of TurrapubinD. [0005] The above-mentioned purpose is achieved through the following technical solutions: [0006] The application of TurrapubinD in the preparation of liver-protecting drugs, the chemical structural formula of TurrapubinD is as follows, [0007] detailed description [0008] Below in conjunction with embodiment furth...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61P1/16
Inventor 林天样
Owner 林天样
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap