Germplasm preservation method of dahlia tissue culture propagation

Abstract

The invention discloses a germplasm preservation method of dahlia tissue culture propagation. The germplasm preservation method comprises the following technical links: 1) selection of an explant and low temperature pretreatment; 2) inoculation; 3) adventitious bud induction and transfer; 4) slow growth and germplasm preservation; 5) rejuvenation; 6) rooting and transplantation. According to the germplasm preservation method of dahlia tissue culture propagation provided by the invention, the germplasm preservation cycle of dahlia is up to 4 years, meanwhile the problems that the occupied area of the germplasm preservation is large, the cost is high and the like are solved, and the operation is simple, the technology is stable and reliable, the repeatability is high, the germplasm preservation method is very suitable for the germplasm preservation of large-scale dahlia, and is an excellent germplasm resource preservation method of the dahlia.

Description

technical field [0001] The invention relates to a germplasm preservation method of dahlia tissue culture propagation, which belongs to the field of agricultural science and technology. Background technique [0002] Dahlia ( Dahlia pinnata Cav) is a perennial bulbous flower of the genus Dahlia in the Asteraceae family. It belongs to about 27 species. It is also known as dahlia chrysanthemum, geranium peony, passion flower, peony, dahlia, sweet potato flower, sweet potato flower, etc. It is native to Mexico, Colombia and Guatemala. and other countries, and later spread to Europe, Japan and my country. Dahlia has thick stems, large leaves, similar flower shape to peony, large and colorful flowers, beautiful flowers, various flower shapes, long flowering period, and various varieties. It is known as the darling of flowers. Dahlia is a natural interspecies hybrid, and the history of artificial breeding is not long. It is widely cultivated in countries all over the world. At pres...

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Problems solved by technology

[0004] At present, most dahlia germplasm resources are preserved by field cultivation, but field cultivation has the following limitations: it is difficult to control light, temperature, water, and pests and diseases, resulting in the loss of germplasm resources; it occupies a large area and consumes manpower, material resources, and Huge financial resources; prone to artificial mixing and species degradation in the long-term asexual reproduction process

At present, there is no mature dahlia germplasm preservation system applied to the industrial development of dahlias. Therefore, in view of the existing shortage of manpower, material resources, space consumption and short preservation time of dahlias, a suitable, long-term preservation, and The low-cost dahlia germplasm resource preservation method is of great significance to the industrial development of dahlia

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