A pretreatment method for microchemical analysis of otoliths

A processing method, the technology of the otolith of the common anchovy, is applied in the pretreatment field of the microchemical analysis of the otolith of the common anchovy, which can solve the problems that the microchemical analysis of the otolith of fish is not applicable, and the heat resistance of the otolith does not meet the analysis requirements, and achieves strong hardness. , good support and high transparency

Active Publication Date: 2018-05-04
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the samples originally involved in the above analytical instruments are mostly metals, minerals, etc. rather than fish otoliths, some original sample pretreatment methods are not suitable for the microchemistry of fish otoliths. analyze
At the same time, the processing methods previously used for microstructure research in ichthyology do not meet the analytical requirements of the above analytical instruments (such as samples need to be polished, inlays need high hardness and certain heat resistance, etc.)

Method used

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  • A pretreatment method for microchemical analysis of otoliths
  • A pretreatment method for microchemical analysis of otoliths
  • A pretreatment method for microchemical analysis of otoliths

Examples

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Embodiment 1

[0035] A method for the pretreatment of the microchemical analysis of the otolith of cuttlefish adopts the following process steps:

[0036] a. Anchovy specimens were collected from the Wuwei section of the Yangtze River in Anhui in early June 2014. After measuring the basic biological data of one of the specimens, the left otolith was removed and washed with deionized water for 3 minutes to remove mucus and envelope , and then use pure alcohol to further clean the organic matter on the surface for 2 minutes, and check whether there is any residual organic matter under a magnifying glass of 10 times, then transfer the otoliths into a 48-well cell culture plate, and dry them at room temperature for 12 hours to obtain otoliths Sample 14WWCE04;

[0037] b. Take 4 ml of AB glue (provided by Japan Konishi Co., Ltd., A glue is epoxy resin, B glue is polythiol curing agent) on a clean aluminum foil, stir for 1 minute, and heat it on an alcohol lamp After 15 seconds to make it watery...

Embodiment 2

[0048] A method for the pretreatment of the microchemical analysis of the otolith of cuttlefish adopts the following process steps:

[0049] a. Anchovy specimens were collected from the Wuwei section of the Yangtze River in Anhui in mid-June 2014. After measuring the basic biological data of one of the specimens, the left otolith was removed and washed with deionized water for 3 minutes to remove mucus and envelope , and then use pure alcohol to further clean the organic matter on the surface for 2 minutes, and check whether there is any residual organic matter under a magnifying glass of 10 times, then transfer the otoliths into a 48-well cell culture plate, and dry them at room temperature for 12 hours to obtain otoliths Sample 14WWCE14;

[0050] b. Take 4 ml of AB glue (Japan Konishi Co., Ltd.) on a clean aluminum foil and stir it for 1 minute, then heat it on an alcohol lamp for 15 seconds to make it watery, and put it on the surface with a diameter of 30 mm and a height o...

Embodiment 3

[0061] A method for the pretreatment of the microchemical analysis of the otolith of cuttlefish adopts the following process steps:

[0062] a. Anchovy specimens were collected from the Wuwei section of the Yangtze River in Anhui in late June 2014. After measuring the basic biological data of one of the specimens, the left otolith was removed and washed with deionized water for 3 minutes to remove mucus and envelope , and then use pure alcohol to further clean the organic matter on the surface for 2 minutes, and check whether there is any residual organic matter under a magnifying glass of 10 times, then transfer the otoliths into a 48-well cell culture plate, and dry them at room temperature for 12 hours to obtain otoliths Sample 14WWCE19;

[0063] b. Take 4 ml of AB glue (Japan Konishi Co., Ltd.) on a clean aluminum foil and stir it for 1 minute, then heat it on an alcohol lamp for 15 seconds to make it watery, and then put it on the surface with a diameter of 28 mm and a he...

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Abstract

The invention relates to a pretreatment method for the microchemical analysis of otoliths of scorpion anchovy, which comprises the following steps: cleaning and drying the otoliths after picking them up, fixing the otoliths on the upper surface of the bottom plate of the otolith fixed mould, preparing reagents, and adding into otolith fixed mold to form cylindrical resin, grinding cylindrical resin, transferring otolith to glass slide, forming otolith slices and drying otolith slices after cleaning; The pretreatment method of chemical analysis, under the premise of satisfying the sample conditions required for microchemical analysis, is combined with the ontological characteristics of fish otolith, a biological tissue. The anchovy otolith samples processed by this processing method can meet various analysis requirements such as electron probe analysis, laser ablation inductively coupled plasma mass spectrometry analysis, stable isotope analysis, etc., and unify the samples required for various otolith microchemical analysis pre-processing method.

Description

technical field [0001] The invention relates to a pretreatment method for microchemical analysis of otoliths of scorpion anchovy, and belongs to the technical field of biological monitoring environment. Background technique [0002] Swordfish ( Coilia nasus ) as one of the "three delicacies of the Yangtze River", has always been an important catch. However, in recent years, due to the impact of overfishing, habitat destruction, and migration passage barriers, its production during the flood season has dropped sharply, and the problem of population miniaturization and younger age is serious. However, as a migratory fish, the complex life history characteristics of the freshwater spawning and hatching and seawater fattening and growth of the anchovy make the environmental history characteristics, migration mode, group origin and relatedness in its migratory ecology delayed. not effectively resolved. Then it brings difficulties to its resource protection, reasonable evaluati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
Inventor 姜涛刘洪波杨健
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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