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Brevibacillus brevis microecological preparation

A technology of Bacillus brevis and micro-ecological preparations, applied in the field of micro-ecological preparations, can solve problems such as susceptible to contamination of miscellaneous bacteria, and achieve the effects of inhibiting the growth of miscellaneous bacteria, solving susceptible to contamination and miscellaneous bacteria, and improving the effect of weight gain.

Active Publication Date: 2015-12-23
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a Bacillus brevis microecological preparation, which can be used to feed animals, and solves the problem that the existing liquid probiotics are easily contaminated with bacteria during preservation, so that it can be stored for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation of embodiment 1 bacillus brevis probiotics

[0019] Streak Bacillus brevis FJAT-0809-GLX (Brevibacillus brevis) on the strain medium with an inoculation loop, and cultivate it in a constant temperature incubator for 24 hours, and the cultivation temperature is 30°C to obtain a single colony; inoculate the obtained single colony Put it in 100ml of seed culture medium, and place it in a constant temperature shaking shaker for 24 hours, the temperature is 30°C, and the rotation speed is 180rpm / min. After the cultivation, the seed liquid is obtained; the seed liquid is put into the fermenter according to the inoculum amount of 1%. Liquid fermentation is carried out in the fermenter, the fermenter is equipped with sterilized fermentation medium, the fermentation parameters are temperature 30 ℃, rotating speed 170rpm / min, air flow 30L / min, fermentation time 48h, then obtain liquid fermented liquid, standby; Take The liquid fermented liquid that obtains, adds N...

Embodiment 2

[0022] The antibacterial activity test of embodiment 2 Bacillus brevis probiotics

[0023] Place the Bacillus brevis probiotics prepared in Example 1 at room temperature in a cool and dry place, and take samples when storing 0d, 10d, 20d, 30d, and 50d respectively; A test tube filled with 9mL of sterile water will make 10 -1 Dilution of the dilution factor, serially diluted to 10 -5 Dilution of the dilution factor) and spread on the NA plate (the components of the NA plate: beef extract 0.3%, peptone 1.0%, sodium chloride 0.5%, agar powder 1.7%, distilled water preparation, pH7.0-7.2), After culturing at 30°C for 48 hours, the number of viable bacteria in each sample was counted, and the antibacterial activity of each sample was detected by the agar diffusion method.

[0024] Test result shows, the Bacillus brevus probiotics prepared in embodiment 1 prolongs along with storage time, viable count and bacteriostatic effect are all more stable, and the viable count of 0d and 30...

Embodiment 3

[0025] Example 3 Effect of Bacillus brevis probiotics on production performance of weaned piglets

[0026] In order to verify the impact of the Bacillus brevis probiotics of the present invention on feeding animals, the applicant conducted the following tests.

[0027] Test location: pig farm in Fuqing National Modern Agriculture Demonstration Park;

[0028] Experimental animals: piglets with a weaning age of 30 days;

[0029]Experimental grouping: 150 weaned piglets of about 5kg were randomly divided into 5 groups (1 as the control group, 4 as the test group, namely the treatment group), each group had 3 columns (repeated), and each replicated 10 pigs, carried out test.

[0030] The conventional ration fed on the pig farm was used as the basal ration, and its composition ratio was shown in Table 1; the feeding scheme of the control group and the test group was shown in Table 2; And the deworming program is carried out according to the pig farm feeding management program; C...

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Abstract

The invention provides a brevibacillus brevis microecological preparation. The brevibacillus brevis microecological preparation is obtained through the following steps: enabling brevibacillus brevis FJAT-0809-GLX (Brevibacillus brevis) to be lined on bacterial strain culture media for culture with an inoculating loop so as to obtain a single colony; inoculating the obtained single colony into seed culture media, and putting the single colony inoculated to the seed culture media in a constant-temperature vibration shaking table for culture so as to obtain seed liquid; pouring the seed liquid according to an inoculum concentration of 1% into a fermenter for performing liquid fermentation so as to obtain liquid fermentation broth for standby application; and taking the liquid fermentation broth, adding NaCl, calcium citrate and xanthan gum, wherein the mass percentage of the NaCl in a solution is 4%, the mass percentage of the calcium citrate in the solution is 0.01%, and the mass percentage of the xanthan gum in the solution is 0.2%, and regulating the pH value to be 7.0-7.2 so as to obtain the brevibacillus brevis microecological preparation. The microecological preparation can be used for feeding animals, and besides, the problem that a conventional liquid microecological preparation is prone to infect with infectious microbes in the storing process, so that the microecological preparation can be stored for a long time.

Description

【Technical field】 [0001] The invention relates to a microecological preparation used in the field of agriculture, in particular to a microecological preparation of Bacillus brevis. 【Background technique】 [0002] In the early 1950s, research found that adding low-concentration antibiotics to feed could significantly promote animal growth, so almost all feed-producing countries in the world used various antibiotics as feed additives, creating the era of antibiotic feed additives. However, with the popularization and application of feed antibiotics, their adverse reactions have gradually become prominent, such as: endogenous infection and cross-infection caused by antibiotics, generation of drug resistance, decreased immunity of livestock and poultry, destruction of normal intestinal flora and Its residues in livestock products and the environment will pose a serious threat to the breeding industry, feed industry and animals, and then endanger human health through the food cha...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/18
Inventor 车建美刘波刘国红陈倩倩唐建阳叶少文
Owner 福建省农业科学院农业生物资源研究所
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