Brevibacillus brevis microecological preparation

A technology of Bacillus brevis and micro-ecological preparations, applied in the field of micro-ecological preparations, can solve problems such as susceptible to contamination of miscellaneous bacteria, and achieve the effects of inhibiting the growth of miscellaneous bacteria, solving susceptible to contamination and miscellaneous bacteria, and improving the effect of weight gain.

Active Publication Date: 2015-12-23
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a Bacillus brevis microecological preparation, which can be used to feed animal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1 Preparation of Bacillus brevis microecological preparation

[0019] Use an inoculation loop to streak Bacillus brevis FJAT-0809-GLX (Brevibacillus brevis) on the strain culture medium, and cultivate it in a constant temperature incubator for 24 hours at a temperature of 30°C to obtain a single colony; inoculate the obtained single colony In 100ml of seed culture medium, place it in a constant temperature shaking shaker for 24h at a temperature of 30°C and a rotation speed of 180rpm / min. After the cultivation, the seed solution will be obtained; the seed solution will be connected to the fermentor at 1% inoculum Liquid fermentation is carried out in the fermentation tank, and sterilized fermentation medium is installed in the fermentation tank. The fermentation parameters are temperature 30℃, rotation speed 170rpm / min, ventilation 30L / min, fermentation time 48h, and then obtain liquid fermentation broth for standby; The obtained liquid fermentation broth is added ...

Example Embodiment

[0022] Example 2 Test of antibacterial activity of Bacillus brevis microecological preparation

[0023] The Bacillus brevis probiotic preparation prepared in Example 1 was stored in a cool and dry place at room temperature, and samples were taken at 0d, 10d, 20d, 30d, and 50d; the samples were serially diluted (1mL sample was drawn to A test tube containing 9mL of sterile water is made into 10 -1 Diluent of dilution multiple, serially diluted to 10 -5 Dilute the dilution times) and spread it on NA plates (components of NA plates: beef extract 0.3%, peptone 1.0%, sodium chloride 0.5%, agar powder 1.7%, distilled water preparation, pH 7.0-7.2), Incubate at 30°C for 48 hours, count the number of viable bacteria in each sample, and use agar diffusion method to detect the antibacterial activity of each sample.

[0024] The test results show that the number of viable bacteria and the antibacterial effect of the Bacillus pumilus microecological preparation prepared in Example 1 are relati...

Example Embodiment

[0025] Example 3 Effect of Brevibacterium Brevibacterium Microecological Preparation on Production Performance of Weaned Piglets

[0026] In order to verify the effect of the Bacillus brevis probiotic preparation of the present invention when it is used to feed animals, the applicant conducted the following tests.

[0027] Test site: pig farm in Fuqing National Modern Agriculture Demonstration Park;

[0028] Test animals: piglets with a weaning age of 30 days;

[0029] Test grouping: Randomly divide 150 weaned piglets of about 5 kg into 5 groups (1 as the control group, 4 as the test group, ie the treatment group), each group has 3 pens (repetitions), and each repeat has 10 pigs. test.

[0030] Regarding the regular diet fed on the pig farm as the basic diet, the composition ratio is shown in Table 1; the feeding plan of the control group and the experimental group is shown in Table 2; the number of test days is 14 days. During the test period, the piglets eat and drink freely and are ...

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Abstract

The invention provides a brevibacillus brevis microecological preparation. The brevibacillus brevis microecological preparation is obtained through the following steps: enabling brevibacillus brevis FJAT-0809-GLX (Brevibacillus brevis) to be lined on bacterial strain culture media for culture with an inoculating loop so as to obtain a single colony; inoculating the obtained single colony into seed culture media, and putting the single colony inoculated to the seed culture media in a constant-temperature vibration shaking table for culture so as to obtain seed liquid; pouring the seed liquid according to an inoculum concentration of 1% into a fermenter for performing liquid fermentation so as to obtain liquid fermentation broth for standby application; and taking the liquid fermentation broth, adding NaCl, calcium citrate and xanthan gum, wherein the mass percentage of the NaCl in a solution is 4%, the mass percentage of the calcium citrate in the solution is 0.01%, and the mass percentage of the xanthan gum in the solution is 0.2%, and regulating the pH value to be 7.0-7.2 so as to obtain the brevibacillus brevis microecological preparation. The microecological preparation can be used for feeding animals, and besides, the problem that a conventional liquid microecological preparation is prone to infect with infectious microbes in the storing process, so that the microecological preparation can be stored for a long time.

Description

【Technical field】 [0001] The invention relates to a microecological preparation used in the field of agriculture, in particular to a microecological preparation of Bacillus brevis. 【Background technique】 [0002] In the early 1950s, research found that adding low-concentration antibiotics to feed could significantly promote animal growth, so almost all feed-producing countries in the world used various antibiotics as feed additives, creating the era of antibiotic feed additives. However, with the popularization and application of feed antibiotics, their adverse reactions have gradually become prominent, such as: endogenous infection and cross-infection caused by antibiotics, generation of drug resistance, decreased immunity of livestock and poultry, destruction of normal intestinal flora and Its residues in livestock products and the environment will pose a serious threat to the breeding industry, feed industry and animals, and then endanger human health through the food cha...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/18
Inventor 车建美刘波刘国红陈倩倩唐建阳叶少文
Owner 福建省农业科学院农业生物资源研究所
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