Application of lonicerin in treating respiratory injury and drug prepared from lonicerin

A technology of respiratory system and luteolin, which is applied in respiratory system diseases, medical preparations containing active ingredients, drug combinations, etc., can solve problems such as inapplicability, and achieve inhibiting weight loss, reducing the incidence of cardiopulmonary diseases, and improving survival. rate effect

Inactive Publication Date: 2015-12-30
PEKING UNIV FIRST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, foreign studies generally obtained dose-response relationships at lower exposure concentrations. However, due to the high exposure concentration of air pollution in China, the existing foreign research results are not applicable to China with severe air pollution.

Method used

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  • Application of lonicerin in treating respiratory injury and drug prepared from lonicerin
  • Application of lonicerin in treating respiratory injury and drug prepared from lonicerin
  • Application of lonicerin in treating respiratory injury and drug prepared from lonicerin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The collection and processing of embodiment 1 atmospheric particulate matter

[0051] Atmospheric particulate matter is collected on the glass fiber filter membrane by the atmospheric particulate matter large-flow sampler of U.S. Staplex Company, and the glass fiber filter membrane that is attached to atmospheric particulate matter is referred to as a small piece, such as in a 250ml beaker; Add sterile ultrapure water therein, Sonicate it for 1 hour at a temperature of 4°C (over 20,000 vibrations per second), during which the beaker is gently shaken every 5 minutes to mix the suspension;

[0052] Pour the suspension in the beaker into a weighed centrifuge tube, wash the filter membrane in the beaker 2-3 times with ultrapure water, transfer all the washing liquid to the centrifuge tube; filter the centrifuge tube with 6 layers of gauze The suspension was freeze-dried at low temperature in a centrifuge tube (-80°C, vacuum, freeze-dried overnight), weighed, and calculated ...

Embodiment 2

[0054] Embodiment 2 The cultivation of alveolar macrophages

[0055] The alveolar macrophages were taken out of the liquid nitrogen and placed in a 37°C water bath to rapidly thaw. Centrifuge immediately after melting, 600-700rpm, 5-6min. Remove the supernatant and add cell culture medium containing 10% fetal bovine serum to resuspend. Put it into a 100mm cell culture dish, and supplement the cell culture medium containing 10% fetal bovine serum to 10ml.

[0056] When the cells grow to more than 90% full, discard the medium, gently wash with phosphate buffer, and add trypsin to digest. After the cells were digested and rounded under the microscope, the trypsin was discarded, and the cell culture medium containing 10% fetal bovine serum was added, all the cells were blown down, centrifuged at a low speed of 500r / min for 2min, and washed with phosphate buffered saline Twice until the trypsin is washed away.

Embodiment 3

[0057] Example 3 The adverse effects of luteolin intervening atmospheric particulate matter on the morphology of alveolar macrophages

[0058] The alveolar macrophages cultured in Example 2 were diluted to 2-3 × 10 6 Cells / mL, the cell dilution solution was divided into 3 mL per dish in cell culture dishes, all placed at 37 ° C, 5% CO 2 Incubate for 4 h in the incubator. After the cells adhered to the wall, they were divided into 3 groups after changing the medium, among which 35 mL of cell culture medium with only 10% fetal bovine serum was added to group A, and 35 mL of cell culture medium with 10% fetal bovine serum was added to group B at a concentration of 100 μg / mL Incubate 2mL of atmospheric particulate matter in group C, add 0.5mL of luteolin at a concentration of 3mg / mL and incubate 2mL of atmospheric particulate matter at a concentration of 100μg / mL in group C. After 24 hours, observe the morphology of alveolar macrophages in different groups under a light microscop...

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Abstract

The invention provides an application of lonicerin in treating respiratory injury caused by atmospheric particulates and a drug prepared from the lonicerin. The drug comprises the lonicerin and a medicinal carrier, wherein the content of the lonicerin in the drug is 60 mg-100 mg / g, the dosage form of the drug is any one of pharmaceutically acceptable dosage forms including tablets, capsules, oral liquids, buccal tablets, granules, pills, powder, sublimed preparation, suspension concentrate, injections and the like. The respiratory injury symptoms include chest distress, cough, expectoration, wheeze and the like. The blank for research of intervention of the lonicerin in harm of the atmospheric particles to human health is filled up, the real drug effect of the lonicerin for intervention of respiratory inflammation is obtained, and the lonicerin also has good effect on diseases of the whole body system.

Description

technical field [0001] The invention relates to the application of luteolin in treating respiratory system damage caused by atmospheric particles and the medicine prepared therefrom, belonging to the field of traditional Chinese medicine / respiratory system diseases. Background technique [0002] As global environmental problems become more and more prominent, the impact of air pollution on the human body, especially the lung toxicity has become the focus of close attention by the government and the public. Large-scale epidemiological studies have shown that acute air pollution exposure is associated with increased mortality in the population. A meta-analysis of Australia, France, and Switzerland found that air pollution kills 40,000 people a year, accounting for 6% of the total annual mortality rate. More than half of this is caused by motor vehicle pollution. In addition, air pollution can cause more than 25,000 new cases of chronic bronchitis in adults, 290,000 cases of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P11/00A61P11/14A61P11/06A61P11/10
Inventor 王广发夏瑞雪
Owner PEKING UNIV FIRST HOSPITAL
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