Unlock instant, AI-driven research and patent intelligence for your innovation.

A magnetic particle chemiluminescence microfluidic chip for quantitative detection of myoglobin

A microfluidic chip and myoglobin technology, which is applied in the field of clinical medical testing, can solve the problems of difficult to achieve micro sample detection, insufficient mixing of the reaction system, complicated microfluidic system, etc., so as to reduce non-specific interference and cost. Low, improve the efficiency of immune response

Active Publication Date: 2017-10-31
SHENZHEN HUAMAIXINGWEI MEDICAL TECH CO LTD
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microfluidic chip system has the characteristics of fast, efficient, and integrated. The combination of the two will become a new type of high-performance detection method to solve the problems of low sensitivity, complicated detection process, and difficulty in achieving trace amounts in current detection methods. The problem of sample detection is expected to further promote the development of clinical testing instruments towards portability and miniaturization
[0006] The biological microfluidic chip of immune magnetic beads is an analysis and detection method that integrates magnetic particle technology and immune analysis on the microfluidic chip. At present, the main difficulties of this comprehensive detection method are as follows: For the intelligent control of internal micro-flow, the method commonly used at present is to set multiple micro-pumps and micro-valves inside the chip, which makes the micro-fluidic system more complicated; 2) Insufficient mixing of the reaction system leads to insufficient reaction; 3) The degree of integration is not high, resulting in high non-specific background

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A magnetic particle chemiluminescence microfluidic chip for quantitative detection of myoglobin
  • A magnetic particle chemiluminescence microfluidic chip for quantitative detection of myoglobin
  • A magnetic particle chemiluminescence microfluidic chip for quantitative detection of myoglobin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: horseradish peroxidase-luminol (HRP-luminol) system is used for the detection of myoglobin

[0055] 1. Fabrication of microfluidic chip

[0056] 1) Antibody labeling: i) Enzyme-labeled antibody: Weigh 5mg of HRP and dissolve in 1ml of distilled water; add 0.2ml of freshly prepared 0.1M NaIO4 solution to the supernatant, and stir for 20 minutes in the dark at room temperature; put the above solution into the dialyzer In the bag, dialyze against 1mM pH4.4 sodium acetate buffer, overnight at 4°C; add 20μl 0.2M pH9.5 carbonate buffer to raise the pH of the above hydroformylated HRP to 9.0-9.5, then immediately add 10mg Myoglobin antibody, in 1ml 0.01M carbonate buffer, stir gently at room temperature for 2 hours in the dark; add 0.1ml of freshly prepared 4mg / ml NaBH4 solution, mix well, and then place at 4°C for 2 hours; put the above solution Put it into a dialysis bag, dialyze against 0.15M pH7.4 PBS, overnight at 4°C; add an equal volume of saturated ammon...

Embodiment 2

[0064] Embodiment 2: alkaline phosphatase-adamantane (ALP-AMPPD) system is used for the detection of myoglobin

[0065] 1. Fabrication of microfluidic chip

[0066]1) Antibody labeling: i) Enzyme-labeled antibody: 2.5mg ALP (50IU / mg), add 200uL 100mM PB (pH6.8) containing 1.25% glutaraldehyde, mix well, and react overnight at room temperature; Electromagnetic stirring, dialyzed to 50mM PBS (pH7.2), 12 hours, change medium 4 times; 1.5mg myoglobin antibody was dissolved in 100uL 1M carbonate solution (pH9.0); activated AP was added to the prepared In protein liquid, mix well, react at 4°C for 24 hours, add 10 μL of 200mM lysine solution, mix well, react at 22°C for 2 hours; dialyze to 50mM PBS (pH7.2) at 4°C, 12 hours, change the medium 4 times; centrifuge, take the supernatant, wash with 50mM TB7.4+0.6%BSA+0.05%NaN 3 Dilute to the desired concentration and store at -20°C. ii) Magnetically labeled antibody: accurately pipette 30 μl of streptavidin-labeled magnetic beads with...

Embodiment 3

[0074] Example 3: Magnetic Particle Size Screening

[0075] The particle size of magnetic microspheres is small, the specific surface area is large, and the surface contains active groups, so the coupling capacity is large, but the size of magnetic particles is too small to be conducive to magnet collection, so the magnetic particle size screening is carried out.

[0076] Refer to Example 2 for other experimental conditions, and the particle size of the magnetic particles is determined according to the following scheme.

[0077] Magnetic particle sizes of 0.1 μm, 0.5 μm, 1.8 μm, 2 μm, 3 μm, and 10 μm were selected to label the anti-C-reactive protein antibody. The permanent magnet whose magnetic size has been optimized is used in the detection to fix the height of the magnet.

[0078] The experimental results are as follows:

[0079] The particle size of magnetic particles increases sequentially from 0.1μm, 0.5μm, 1.8μm, 2μm, and 3μm. The interference increases at 3μm and de...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
volumeaaaaaaaaaa
widthaaaaaaaaaa
depthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a magnetic particle chemiluminescent microfluidic chip for quantitative detection of myoglobin, the structure of which mainly includes a cover (1) and a negative (11), wherein an air pump (3) on the cover (1), The airflow microchannel (5), the sample injection port (2), the sample liquid flow channel (6), the first biomarker storage pool (4), the micromixer (7) and the transition zone (10) are connected in sequence; the negative film ( The filter (12) on the 11), the reaction tank (13), the cleaning tank (14), the detection tank (15), and the solution release channel (18) are connected successively; the detection tank (15) on the negative film (11) passes through the solution The release channel (18) is connected with the cleaning liquid storage pool (16) and the luminescence liquid storage pool (17).

Description

technical field [0001] The invention relates to the field of clinical medical examination, in particular to a magnetic particle chemiluminescence microfluidic chip for quantitative detection of myoglobin, which can realize quantitative detection of myoglobin in biological samples within a few minutes, and the detection method has the advantages of simple operation , high sensitivity, low cost and so on. technical background [0002] Myoglobin (MYO, Mb) is a binding protein composed of a peptide chain and a heme prosthetic group. It is a protein that stores oxygen in muscle. Its oxygen saturation curve is hyperbolic. Oxygen-carrying protein in muscle, consisting of 153 amino acid residues, molecular weight 16.7KD, containing heme, homologous to hemoglobin, and oxygen-binding ability between hemoglobin and cytochrome oxidase, which can help muscle cells convert Oxygen transport to mitochondria. Myoglobin is the main protein that composes skeletal muscle and cardiac muscle. W...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N21/76G01N33/553
Inventor 范玉霞李泉
Owner SHENZHEN HUAMAIXINGWEI MEDICAL TECH CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com