The invention discloses a biological enzyme fracturing technology for oil fields and oil wells. The technology comprises the following steps of: connecting a ground pipeline, testing the pressure of a high-pressure pipeline, backwashing a well, starting a pump for fracturing till a fracturing pressure is displayed, and starting injection after the pressure is stable, wherein the injection has the sequence of: injecting an ahead fluid, injecting a base fluid, adding a cross-linking agent and a propping agent at the same time of injecting the base fluid, injecting a gel breaker, closing the mounting wellhead to build the pressure, controlling blow-out of a pressure oil pipe, and pulling out a shaft pipe column after the pressure of the wellhead is dispersed. The technology solves the problems of cold damage, water damage and residue damage, and has the advantages of good environmentally-friendly effect, wide application range, high compatibility, thorough gel breakage, simplicity in operation and high cost performance.