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Pure yessotoxin (YTX) extracting and preparing method

A technology of scallop toxin and pure product, which is applied in the direction of organic chemistry, can solve the problems of not being able to meet the needs of preparing YTX standard products, not involving specific steps and methods for YTX purification, and being unsatisfactory, so as to achieve the goal of promoting marine food safety The effect of work

Inactive Publication Date: 2015-12-30
NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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  • Application Information

AI Technical Summary

Problems solved by technology

In the current reports, the extraction of YTX is mainly used for property analysis, the extracted amount is very small, and the purity is not high, which cannot meet the needs of preparing YTX detection standards
At the same time, the existing literature does not involve the specific steps and methods of YTX purification, which cannot meet the needs of preparing YTX standards

Method used

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  • Pure yessotoxin (YTX) extracting and preparing method
  • Pure yessotoxin (YTX) extracting and preparing method
  • Pure yessotoxin (YTX) extracting and preparing method

Examples

Experimental program
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Embodiment Construction

[0024] The present invention will be further explained below in conjunction with specific examples.

[0025] 1. Methanol crude extraction

[0026] 1. Add an appropriate amount of methanol to a 50mL centrifuge tube containing 0.5g algae cake, stir well with a glass rod, ultrasonically extract for 15min, 3500r / min, centrifuge for 10min, collect the supernatant, add an appropriate amount of methanol to the residue, and centrifuge repeatedly 1. Collect the supernatant 3 times. All the obtained supernatant was placed overnight in a -80°C refrigerator, and the next day, the extract was filtered with a filter membrane with a pore size of 0.45um, and the filtrate was collected.

[0027] 2. Rotate the above filtrate to evaporate (100rpm / min, 40°C). After evaporating to dryness, add 12ml of methanol in 3 times, and use an elbow plastic dropper to continuously rinse from the neck of a flat-bottomed rotary evaporator (25mL) , and then sonicate until the solute on the bottle wall is comp...

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Abstract

The invention discloses a pure yessotoxin (YTX) extracting and preparing method. YTX samples prepared through the steps of coarsely extracting YTX through methyl alcohol, twice separating and extracting YTX, preparing pure YTX and the like can be used for manufacturing YTX standard products and measuring the content of YTX in sea food such as fish, shrimp and shellfish, and the content of YTX belongs to one of indexes which must be detected in the field of sea food safety. By means of the method, the blank that YTX standard products are lacked in China is filled in, the development of the YTX detection technology in China and the work of sea food safety are greatly promoted, and a great promotion effect is achieved for making sea products in China smoothly exported to Europe and other developed countries.

Description

technical field [0001] The YTX sample prepared by the invention can be used to make YTX toxin standard products and determine the content of scallop toxin YTX in marine food (fish, shrimp, shellfish, etc.), which belongs to one of the indicators that must be detected in the field of marine food safety. Background technique [0002] In the prior art, foreign literatures have reported research on YTX toxin, which is mainly used for the research of YTX toxin, structure characterization, chemical structure determination of derivatives of YTX toxin, and the relationship between YTX toxin production environment and toxin production. There is no specific research on the chemical molecular structure and properties of YTX toxin in the domestic literature. [0003] The preparation of the YTX standard product requires that the obtained YTX product must have a certain yield, and at the same time, the purity must meet certain requirements. In the current reports, the extraction of YTX i...

Claims

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Application Information

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IPC IPC(8): C07D493/22
CPCC07D493/22
Inventor 杨琳梁玉波刘仁沿刘磊许道艳李冬梅
Owner NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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