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Fast detection method of leather-hydrolyzed protein L-hydroxyproline

A hydroxyproline and detection method technology, which is applied in the field of rapid detection of leather milk hydrolyzed protein L-hydroxyproline, can solve the problems of cumbersome operation and high toxicity of detection reagents, and achieve cumbersome and simple operation, less sampling volume, and high-tech simple effect

Inactive Publication Date: 2015-12-30
SHAANXI INST FOR FOOD & DRUG CONTROL +1
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AI Technical Summary

Problems solved by technology

Colorimetric detection reagents are highly toxic and relatively cumbersome to operate

Method used

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Embodiment

[0014] This embodiment mainly introduces the preparation method of the solution and reagent used in the present invention and the flow method of the detection operation.

[0015] 1) Preparation of pretreatment reagents: Accurately weigh 219g of zinc acetate and 30mL of glacial acetic acid, dilute to 1000mL with distilled water, and stir to dissolve. Accurately weigh 106g of potassium ferrocyanide, dilute to 1000mL with distilled water, and stir to dissolve.

[0016] 2) Preparation of oxidation buffer solution: Weigh 6.95 g of anhydrous disodium hydrogen phosphate, 8 g of sodium dihydrogen phosphate dihydrate, and 7.05 g of chloramine T, and dilute to 500 mL with distilled water, and stir to dissolve.

[0017] 3) Preparation of color developer: Accurately measure 100mL of concentrated hydrochloric acid and slowly add it to 100mL of water; weigh 50g of p-dimethylaminobenzaldehyde, measure 175mL of the diluted concentrated hydrochloric acid solution, stir to dissolve, and then ad...

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Abstract

The invention discloses a fast detection method of leather-hydrolyzed protein L-hydroxyproline, in particular to a fast detection method of leather-hydrolyzed protein L-hydroxyproline illegally added into milk. The fast detection method of the leather-hydrolyzed protein L-hydroxyproline illegally added into the milk is convenient to use, fast, simple and convenient to operate and high in sensitivity and is characterized in that a reagent is added into the milk or a dairy product to be detected and is heated in water bath to conduct precipitation treatment on protein in the milk or the dairy product, and standing is performed for layering; an oxidizing agent and a color developing agent are added into supernatant collected in the step 1, reaction is performed for a period of time, and then a colorimetric method is adopted to determine the L-hydroxyproline so as to obtain related results.

Description

technical field [0001] The invention belongs to the technical field of spectrophotometry and relates to a detection method, in particular to a rapid detection method of leather milk hydrolyzed protein L-hydroxyproline. Background technique [0002] The so-called "leather hydrolyzed protein" is a kind of protein powder produced by hydrolysis technology by using leather leftovers or animal fur, organs, etc. Like melamine, it can be mixed with milk or milk powder to increase the protein content. The Ministry of Agriculture's detection of "leather hydrolyzed protein" in fresh dairy products did not start this year. After the "melamine milk" incident, the Ministry of Agriculture began to monitor the quality and safety of fresh milk nationwide in 2009. The monitoring content included melamine, leather hydrolyzed protein and alkali substances. On January 5, 2011, the Ministry of Agriculture The "2011 Fresh Dairy Products Quality and Safety Monitoring Plan" was issued, and the ite...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/28G01N1/38
Inventor 刘海静李涛马魁乔蓉霞林芳王宇冯润东裴小龙王一欣
Owner SHAANXI INST FOR FOOD & DRUG CONTROL
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