A preparation method of magnetic particles for tumor targeting therapy

A magnetic particle and tumor-targeting technology, applied in the field of medical materials, can solve the problems of unsafe reduction of "particle agglomeration, negative effects of polymer viscosity, unfavorable drug combination and adhesion, etc., to save time and cost, reduce production cost, performance improvement

Active Publication Date: 2018-07-20
周洪珍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After surface modification by SAA, CNTs exhibit good dispersibility and processability, as mentioned above, but when used in the field of gene therapy, it is necessary to combine drugs and polymer materials, and use SAA-modified Fe 3 o 4 Magnetic microspheres, ①It has a negative effect on the viscosity of the polymer, which is not conducive to the binding and attachment of drugs; ②It is necessary to repeatedly verify whether SAA and drug activity conflict, and Fe after the same SAA modification 3 o 4 Magnetic microspheres cannot be used for all drug carriers; ③The in vivo toxicity of surfactants must be fully evaluated and carefully selected; ④The cost of SAA modification is high
[0005] In summary, many steps and the inability to safely reduce the "particle agglomeration" in the synthesis process are the biggest disadvantages of the current synthesis method

Method used

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  • A preparation method of magnetic particles for tumor targeting therapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Weigh 2 moles of NaOH, dissolve in deoxygenated pure water to form a 1.0mol / L solution, heat to 60°C, add 40g of soluble starch, stir until completely gelatinized, and form a mixed solution; add the mixed solution to Put into the reaction kettle, seal the reaction kettle, use high-purity nitrogen to purge the reaction kettle through the inlet valve, and discharge the internal air; because the soluble starch is added to the hot NaOH solution for gelatinization reaction, the starch molecules in the granules stretch in all directions Diffusion and dissolution out of the granules, the expanded starch molecules will be connected and entangled with each other to form a network of hydrocolloids. When the starch enters the granule disintegration stage of the gelatinization reaction, the viscosity of the solution is the largest, which enables the starch molecules to wrap around NaOH, inhibits the reaction speed of NaOH with iron ions and ferrous ions, and inhibits the formati...

Embodiment 2

[0034] (1) Weigh 8 moles of NaOH, dissolve in deoxygenated pure water to form a 1.5mol / L solution, add 120g of water-soluble starch, and stir until completely dissolved at room temperature; form a mixed solution; add the mixed solution to the reaction kettle , seal the reactor, use high-purity nitrogen to purge the reactor through the inlet valve, and discharge the internal air;

[0035] (2) Weigh 1.2 moles of FeCl 3 ·6H 2 O, 1 mole FeCl 2 4H 2 O is dissolved in deoxygenated pure water to form a mixed solution; its concentration is 1.2mol Fe per liter of solution 3+ and 1.0mol Fe 2+ .

[0036] (3) drop the mixed solution of step (2) gained in step (1) gained sodium hydroxide starch gelatinization solution by feed valve. The ratio of raw materials added is molar ratio OH — : Fe 3+ : Fe 2+ =8:1.2:1, heat the reactor to 200°C, keep it warm for 8 hours, after cooling down naturally, wash and dry to obtain carbon-coated nano-magnetic Fe 3 o 4 .

[0037] In this embodime...

Embodiment 3

[0039] (1) First weigh 8 moles of NaOH, dissolve it in deoxygenated pure water to form a 1.2mol / L solution, add 80g of pregelatinized starch at 40°C, and stir until completely dissolved; ultrasonically mix at a frequency of 200Hz, and ultrasonically Make the air bubbles in the mixture vibrate under the action of sound waves, and when they reach a certain limit, they will grow and collapse to play a role of cavitation. Using ultrasonic cavitation, NaOH and starch molecules in the solution are evenly mixed and the size of starch particles is controlled. evenly distributed. Add the mixed solution into the reaction kettle, seal the reaction kettle, use high-purity nitrogen to purge the reaction kettle through the inlet valve, and discharge the internal air;

[0040] (2) Weigh 1.5 moles of FeCl 3 ·6H 2 O, 1 mole FeCl 2 4H 2 O is dissolved in deoxygenated pure water to form a mixed solution; its concentration is 1.5mol Fe per liter of solution 3+ and 1.0mol Fe 2+ .

[0041] (...

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Abstract

The invention discloses a preparation method of magnetic granules for targeting therapy of tumor and belongs to the field of medical polymer materials and provides the preparation method to solve the defects that a coating process of magnetic granule carriers for traditional targeting therapy is complex, production cost is high and product performance is unstable. The preparation method is characterized in that sodium hydroxide starch paste and iron-containing solution are mixed and reacted in a high-temperature reactor in an inert atmosphere, reaction temperature and time are under control, and performing filtering, washing, drying and screening after reacting to obtain carbon-coated ferroferric oxide. Compared with the prior art, the preparation method finishes carbon coating during hydro-thermal synthesis and has the advantages of process simplicity, low cost, uniformity of carbon-coated ferroferric oxide granularity, and uniformity of carbon coating thickness.

Description

technical field [0001] The invention relates to medical materials, in particular to a method for preparing a carbon-coated ferric oxide nanometer material for targeted therapy. Background technique [0002] Targeted tumor therapy is to design corresponding therapeutic drugs for the identified cancer-causing site (the site can be a protein molecule or a gene fragment inside the tumor cell) at the molecular level of the cell, and the drug enters The body will specifically select oncogenic sites to combine and act, so that the tumor cells will die specifically, without affecting the normal tissue cells around the tumor, so molecular targeted therapy is also called "bio-missile". The development of new vectors is a key issue in enhancing therapeutic targeting. The development of nanotechnology has provided a new idea for solving the problem of targeted transfer vectors. Magnetic nanoparticles with small size and superparamagnetism are expected to become ideal gene carrier mate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/02A61P35/00
Inventor 周洪珍
Owner 周洪珍
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