A method for extracting plant calcium from soybean seedlings
A technology for soybean seedlings and plant calcium, which is applied in the field of plant calcium extraction, can solve the problems of difficult absorption and easy formation of stones, and achieve the effects of good absorption effect, easy consumption, and high calcium ion content
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Embodiment 1
[0020] In the embodiment of the present invention, a method for extracting plant calcium from soybean seedlings consists of the following steps:
[0021] 1) First remove the damaged part of the fresh soybean seedlings collected, rinse with clean water, soak in 70% alcohol solution, then drain the alcohol solution on the screen, and put it in sterile water to wash away the alcohol solution , Drain on the screen. Wash again with sterile water to ensure that no alcohol solution remains.
[0022] 2) In order to reduce the contamination of microorganisms, the soybean seedlings cleaned with alcohol are immediately sent to the aseptic working room and crushed with a vegetable grinder. Cellulase and pectinase are added to the crushed soybean seedlings at an ambient temperature of 45°C. Keep the temperature for 20 minutes, in which the addition of cellulase and pectinase is 1% of the total mass of soybean seedlings.
[0023] 3) Add hydrochloric acid to the above-mentioned enzyme-treated sol...
Embodiment 2
[0027] In the embodiment of the present invention, a method for extracting plant calcium from soybean seedlings consists of the following steps:
[0028] 1) First remove the damaged parts of the fresh soybean seedlings collected, rinse them with clean water, soak them in a 75% alcohol solution, then drain the alcohol solution on the screen, and put it in sterile water to wash away the alcohol solution , Drain on the screen. Wash again with sterile water to ensure that no alcohol solution remains.
[0029] 2) In order to reduce the contamination of microorganisms, the soybean seedlings cleaned with alcohol are immediately sent to the aseptic working room and crushed with a vegetable grinder. Cellulase and pectinase are added to the crushed soybean seedlings at an ambient temperature of 55°C Keep the temperature for 40 minutes, in which the addition of cellulase and pectinase is 5% of the total mass of soybean seedlings.
[0030] 3) Add hydrochloric acid to the above-mentioned enzyme...
Embodiment 3
[0034] In the embodiment of the present invention, a method for extracting plant calcium from soybean seedlings consists of the following steps:
[0035] 1) First remove the damaged parts of the fresh soybean seedlings collected, rinse with clean water, soak in 72% alcohol solution, then drain the alcohol solution on the screen, and put it in sterile water to wash away the alcohol solution , Drain on the screen. Wash again with sterile water to ensure that no alcohol solution remains.
[0036] 2) In order to reduce the contamination of microorganisms, the soybean seedlings cleaned with alcohol are immediately sent to the aseptic working room and crushed with a vegetable grinder. Cellulase and pectinase are added to the crushed soybean seedlings at an ambient temperature of 50℃. Keep the temperature for 30 minutes, where the addition of cellulase and pectinase is 3% of the total mass of soybean seedlings.
[0037] 3) Add hydrochloric acid to the above-mentioned enzyme-treated soluti...
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