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A kind of multifunctional bacteria fermentation compound enzyme feed and preparation method thereof

A bacterial fermentation and multi-functional technology, applied in the field of multi-functional bacterial fermentation compound enzyme feed and its preparation, can solve the problems of instability of compound enzyme fermentation feed, failure to achieve expected results, backward fermentation technology, etc., and reduce intestinal diseases. , The effect of improving the flora environment and inhibiting reproduction

Active Publication Date: 2019-12-10
李德舜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, our country's compound enzyme fermented feed also has shortcomings such as single strains and backward fermentation technology, which has caused the instability of our country's compound enzyme fermented feed and failed to achieve the expected effect.

Method used

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  • A kind of multifunctional bacteria fermentation compound enzyme feed and preparation method thereof
  • A kind of multifunctional bacteria fermentation compound enzyme feed and preparation method thereof
  • A kind of multifunctional bacteria fermentation compound enzyme feed and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of multifunctional bacteria fermentation compound enzyme feed

[0028] 1. Fermentation strains:

[0029] Aspergillus basilica is a strain with the number 3.7885 of the China General Microorganism Culture Collection Management Center; Paelopsis is a strain with the preservation number CICC 31258 of the China Industrial Microbiology Culture Collection Management Center; Bacillus subtilis is a China Industrial Microbiology Culture Collection The preservation number of the management center is the strain of CICC 21312; the probiotics are Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus casei mixed at a mass ratio of 1:1:1:1, wherein Streptococcus thermophilus Cocci, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus casei can all be purchased; yeast and photosynthetic bacteria are commercial products.

[0030] 2. Medium:

[0031] PDA liquid medium: Peel 200g of potatoes, cook i...

Embodiment 2

[0043] Embodiment 2: Multifunctional bacteria fermentation compound enzyme feed related index detection

[0044] Indexes such as crude protein, crude fat, crude fiber, free amino acids and compound enzymes in the multifunctional bacteria fermentation compound enzyme feed prepared in Example 1 are detected, with unfermented feed as a comparison, the results are shown in Table 1-3.

[0045] Table 1 Test results of sample indicators before and after fermentation

[0046]

[0047] Table 2 Analysis of free amino acids in samples before and after fermentation

[0048]

[0049]

[0050] Table 3 Feed compound enzyme analysis before and after fermentation

[0051]

[0052] As can be seen from Table 1-3, the content of crude protein and crude fat in the multifunctional bacterium fermentation compound enzyme feed of the present invention obviously improves, and the content of multiple free amino acids also improves, and amylase, cellulase, acid Protease and xylanase have al...

Embodiment 3

[0053] Example 3: Verification of deodorizing effect of multifunctional bacteria fermented compound enzyme feed

[0054] Selecting body weight is 80 of Du Chang'an healthy pigs about 40Kg, is divided into 2 groups at random (comprising 1 test group and 1 control group), test group 1 gives the multifunctional bacterium fermentation compound protease feed that embodiment 1 prepares, contrast The pigs in each group were given conventional commercially available pig feed; the test pigs in each group were allocated to the same pig house in two houses separated by partitions, gas non-convection, relatively independent, and with the same environment. , each repeated 10 pigs, the test pigs were free to eat, and the test period was 30 days.

[0055] The feces just excreted were sampled before the test (0th day), 7th day, 14th day, 21st day, and 28th day, and the feces were fully mixed on site, and the samples were processed immediately after being brought back to the laboratory for vul...

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Abstract

The invention discloses multifunctional bacterium fermented compound enzyme feed. A bacterium solution compounded by aspergillus albicans, endomycopsis and bacillus in the volume ratio being (1-2):(2-3):(4-6) is mixed with feed, the mixture is fermented, then a bacterium solution compounded by yeast, probiotics and photosynthetic bacteria in the volume ratio being (2-3):(4-6):(2-3) is added to the fermented material, and anaerobic fermentation is performed for preparation; the feed is mixed by bran, soybean cake powder and mushroom residues in the mass ratio being (50-70):(10-20):(10-20). The invention further discloses a preparation method of the compound enzyme feed. The compound enzyme feed is fermented by various bacteria, so that not only acid protease, neutral protease and cellulase are produced, but also the protein content of the feed is increased remarkably, and peculiar smell of livestock excrement can be eliminated effectively.

Description

technical field [0001] The invention relates to a multifunctional bacteria fermented composite enzyme feed and a preparation method thereof. Background technique [0002] Compound enzymes for feed are a class of hydrolytic enzymes, including amylase, protease, cellulase and other enzymes. A considerable part of the organic matter in livestock supplements cannot be digested, and the addition of compound enzymes can supplement the deficiency of animal endogenous enzymes, eliminate anti-nutritional factors in feed, significantly improve the utilization rate of feed, expand the range of available feed resources, reduce Reduce the cost of breeding and improve the ecological environment of breeding. [0003] In recent years, the application of enzymes in the feed industry has achieved rapid development, and good results have been achieved in the application of livestock and poultry. According to reports, pigs and chickens gain weight between 5-40%, save feed by 10-30%, and can r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/30
Inventor 李德舜李瑾刘博
Owner 李德舜
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