Nucleic acid amplification reaction apparatus and control method therefor
A technology of nucleic acid amplification reaction and control method, applied in the field of nucleic acid amplification reaction devices, capable of solving problems such as instability and variation of nucleic acid amplification reaction
Inactive Publication Date: 2016-01-27
SEIKO EPSON CORP
View PDF1 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
At this time, since the high-temperature oil rises and the low-temperature oil falls, convection occurs in the oil in the container, the temperature of the oil at both ends of the container fluctuates, and the nucleic acid amplification reaction becomes unstable.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0017] Hereinafter, preferred embodiments of the present invention will be described in the following order using the drawings. In addition, the embodiment described below does not unreasonably limit the content of the present invention described in the claims. In addition, not all the configurations described below are necessarily essential components of the present invention.
[0018] 1. Structure of nucleic acid amplification reaction device
[0019] FIG. 1 is a perspective view of a nucleic acid amplification reaction device 1 according to one embodiment of the present invention. Figure 1A Indicates the state in which the cover 50 of the nucleic acid amplification reaction device 1 is closed, Figure 1B It shows the state where the cover 50 of the nucleic acid amplification reaction apparatus 1 is opened, and also shows the state where the nucleic acid amplification reaction container 100 is mounted on the mounting part 11 . figure 2 It is an exploded perspective view ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention provides a nucleic acid amplification reaction apparatus and a control method therefor. The nucleic acid amplification reaction apparatus includes a first heating section configured to heat a first region of a nucleic acid amplification reaction container having a longitudinal direction and filled with reaction liquid and liquid that has specific gravity smaller than the specific gravity of the reaction liquid and does not mix with the reaction liquid and a second heating section configured to heat a second region of the nucleic acid amplification reaction container to temperature higher than the temperature of the first heating section, a first arrangement is an arrangement in which the first region is below the second region in a direction in which the gravity acts and a second arrangement is an arrangement in which the second region is below the first region in the direction in which the gravity acts. A third arrangement is an arrangement in which an axis in the longitudinal direction of the nucleic acid amplification reaction container is horizontal or an arrangement in which the axis inclines with respect to the vertical line and the second region is below the first region in the direction in which the gravity acts. A driving mechanism is controlled to switch the first arrangement, the second arrangement, and the third arrangement.
Description
technical field [0001] The invention relates to a nucleic acid amplification reaction device and a control method thereof. Background technique [0002] As a method for amplifying nucleic acid at a high speed, a method is known in which oil and a nucleic acid amplification reaction solution that is not mixed with the oil and has a different specific gravity are put into a nucleic acid amplification reaction container, one end of the container is maintained at a high temperature, and the other end is kept at a high temperature. One end is maintained at a low temperature, and the container is rotated to alternately switch between a state in which the reaction solution is placed in high-temperature oil with one end of the nucleic acid amplification reaction container vertically downward, and a state where the other end of the nucleic acid amplification reaction container The nucleic acid amplification reaction solution was thermally cycled by placing the reaction solution in lo...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): C12M1/38
CPCB01L7/52B01L2300/1805B01L7/525B01L2200/0673B01L2200/14B01L2200/143B01L2300/0832B01L2400/0457B01L7/5255G01N35/026G01N2035/00366
Inventor 富樫健高城富美男村山寿郎
Owner SEIKO EPSON CORP