Method for screening of neuropeptide receptors

A neuropeptide and receptor technology, applied in special data processing applications, instruments, electrical digital data processing, etc., can solve the problems of many interference factors, difficulty in determining receptors, heavy workload, etc., and achieve good accuracy and repeatability , reduce laboratory work, save experimental costs

Inactive Publication Date: 2017-12-26
SHANXI AGRI UNIV
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Problems solved by technology

[0005] At present, the screening of neuropeptide receptors mostly adopts traditional large-scale screening methods, such as yeast two-hybrid, bacterial two-hybrid, protein chip and immunoaffinity technology. The problems of false positive and false negative in the results are prominent, and there are many interference factors, which make it difficult to determine the receptors in the later stage

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  • Method for screening of neuropeptide receptors
  • Method for screening of neuropeptide receptors
  • Method for screening of neuropeptide receptors

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Embodiment 1

[0031] The method of the present invention is used to screen the candidate receptors of bovine follicle cocaine-and amphetamine-regulated transcription peptide (CART), and adopt the following steps:

[0032] S1. Eight 10-month-old young cows (Hyford hybrid offspring) were selected and treated in estrus during the same period. During this period, the growth status of the follicles was detected and recorded with a B-ultrasound instrument every day. When the follicle development deviation phase of the first follicle wave starts, the ovaries are slaughtered and removed, and the largest follicle ODF1 (The largest follicle at onset of deviation, 4~5d after estrus) and the second largest follicle when the deviation occurs are cut with ophthalmic scissors. Follicle ODF2 (The second largest follicle atonset of deviation), put it in sterilized DPBS for use;

[0033] S2. Cut a small mouth of the follicle obtained from S1 with ophthalmological scissors and place it on a watch glass containing ...

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Abstract

The invention discloses a method for screening neuropeptide receptors. According to the principle of statistics, P<0.05 after FDR correction of genome sequencing results is required, and the selection basis of gene differential expression multiples is the effect produced by ligand-receptor binding. The strength is proportional to the number of occupied receptors; the protein model is established by using protein homology modeling technology; the three homology modeling technologies of SWISS‑MODEL, MODELLER and PHYRE2 with the most comprehensive evaluation system are comprehensively applied to avoid the single modeling technology The quality of the model is not high; this specific implementation is in the process of molecular docking, the setting requirements of each technical parameter are clear, and the docking area is accurate. After the docking is successful, the docking needs to be optimized by geometric matching and molecular dynamics methods. The molecular force field evaluation function was used to obtain the docking complex score, and the candidate receptors were determined by comprehensive expression verification, molecular characteristics and functional analysis of neuropeptide receptors.

Description

Technical field [0001] The invention relates to the technical field of neuropeptide biology and animal physiological regulation and drug target screening research, and specifically relates to a method for neuropeptide receptor screening. Background technique [0002] The study of receptor interaction and regulation mechanism opened up new ideas. As an important chemical messenger between neurons, neuropeptides perform the functions of neuromodulators, neurotransmitters and neurohormones. Neuropeptides are widely distributed in animals, indicating the diversity and complexity of biological functions, which are mainly reflected in: 1) a large number of neuropeptides; 2) the same neuropeptide has multiple functions, and it can affect the same target organ (target tissue or target). Cells) have different effects; 3) The same neuropeptide has different functions depending on the species, dosage, and site of action; 4) The same neuropeptide has different effects on different effector ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06F19/16
Inventor 李鹏飞吕丽华谢建山孟金柱刘岩姚晓磊赵妙妙景炅婕毕锡麟王锴
Owner SHANXI AGRI UNIV
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