Molecular imprinting piezoelectric sensor for detecting trace citrinin and preparation method of molecular imprinting piezoelectric sensor

A piezoelectric sensor and molecular imprinting technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of limited imprinting sites, achieve long service life, high sensitivity and accuracy, and easy control of reaction conditions

Inactive Publication Date: 2016-02-17
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, because the molecularly imprinted polymer membranes prepared by electropolymerization are usually dense, the effective imprinted sites are limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method of a molecularly imprinted piezoelectric sensor for detecting trace amounts of citrinin, the steps of the preparation method are:

[0023] (1) Add 1 mL of 1% chloroauric acid solution to 99 mL of double-distilled water, stir evenly, and heat. When the solution boils, quickly add 2.25 mL of 1% trisodium citrate solution to it. As the reaction progressed, the color of the solution gradually changed from yellow to wine red. After the solution completely turned wine red, heating was continued for 15 min, then the heating was stopped, and stirring was continued until the solution was cooled to room temperature. Finally, distill the solution to 100 mL with double-distilled water to obtain a colloidal gold solution, which is stored at 4°C in the dark. 30 mg of mesoporous carbon CMK-3 was added to 100 mL of the above colloidal gold solution, stirred for 4 h, and the obtained gold nanoparticle mesoporous carbon CMK-3 composite was collected after centrifugat...

Embodiment 2

[0030] The above-mentioned molecularly imprinted piezoelectric sensor for detecting trace amounts of citrinin is tested for citrinin in actual samples:

[0031]Citrinin in actual samples (rice, wheat, rice vinegar) was analyzed and determined by using the sensor of the present invention and high performance liquid chromatography respectively, and no citrinin was detected. The standard addition method was used to carry out the standard addition recovery experiment, and the sensor of the present invention was used to measure in parallel three times at three concentrations, and the calculated recovery rate was 85.8% to 93.1%.

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Abstract

The invention provides a molecular imprinting piezoelectric sensor for detecting trace citrinin and a preparation method of the molecular imprinting piezoelectric sensor. A composite film on the surface of an electrode of the sensor is gold nanoparticle@mesoporous carbon CMK-3 composite and a molecular imprinting polymer, the gold nanoparticle@mesoporous carbon CMK-3 composite film has a large specific surface area and a three-dimensional ordered mesoporous structure, and the molecular imprinting polymer can provide molecular imprinting holes corresponding to citrinin molecules. During preparation, a quartz crystal gold electrode is coated with the gold nanoparticle@mesoporous carbon CMK-3 composite and then put into an electrolyte solution containing dummy templates and functional monomers to have an electrochemical polymerization reaction, the dummy templates are removed after the reaction ends, and the molecular imprinting piezoelectric sensor is obtained and used for detecting citrinin. The preparation method is simple, the cost is low, and the molecular imprinting piezoelectric sensor has good selectivity, repeatability and anti-interference capacity and is successfully applied to detection of citrinin in an actual sample.

Description

technical field [0001] The invention relates to a piezoelectric sensor based on molecular imprinting technology, in particular to a molecularly imprinted piezoelectric sensor for detecting trace amounts of citrinin and a preparation method thereof. Background technique [0002] Citrinin (Cit) is a toxic secondary metabolite produced by some filamentous fungi, Monascus, Aspergillus and Penicillium, and often contaminates grains, food, feed, biological fluids, etc. In addition to strong nephrotoxic effects, studies have found that citrinin also has carcinogenic, mutagenic and teratogenic effects. So far, the qualitative and quantitative methods of citrinin mainly include high performance liquid chromatography, thin layer chromatography, enzyme-linked immunosorbent assay, liquid chromatography-mass spectrometry, and inhibition zone method. However, due to the poor stability of citrinin in food and the lack of suitable and accurate analytical methods, there is currently no unif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N30/02
CPCG01N27/30G01N30/02G01N2030/027
Inventor 方国臻刘桂洋王俊平王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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