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A method for determining the content of scutellarein and scutellarein in Scutellaria barbata or its formula granules

A technology of scutellarein and scutellarein, which is applied in the field of content determination of scutellarein and scutellarein in Scutellaria barbata or its formula granules, which can solve the problems of unchecked and ensure accuracy and reproducibility Effect

Active Publication Date: 2017-06-30
HEBEI UNIV OF CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the simultaneous determination of scutellarin and scutellarein by isocratic elution

Method used

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  • A method for determining the content of scutellarein and scutellarein in Scutellaria barbata or its formula granules
  • A method for determining the content of scutellarein and scutellarein in Scutellaria barbata or its formula granules
  • A method for determining the content of scutellarein and scutellarein in Scutellaria barbata or its formula granules

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Chromatographic conditions and system suitability test Acetonitrile-methanol-0.1% phosphoric acid with a volume ratio of 12.5:15:72.5 is used as the mobile phase; column temperature: 30°C; detection wavelength is 340±2nm; Glycoside peak calculation should not be less than 3000;

[0045] (2) Preparation of reference substance solution Take appropriate amount of scutellarein and scutellarein reference substance respectively, accurately weigh them, put them in a brown measuring bottle, add 70% methanol to make each 1ml contains 40 μg of scutellarin and 12 μg of scutellarein The solution, as the reference substance solution;

[0046] (3) Preparation of the test solution Take 0.5g of Scutellaria barbata medicinal material fine powder, accurately weighed; or 0.1g of Scutellaria barbata formula granules, accurately weighed; respectively put in conical flasks with stoppers, and accurately add 70% methanol 25ml, densely packed, weighed, the fine powder of medicinal material...

Embodiment 2

[0049] (1) Chromatographic conditions and system suitability test Acetonitrile-methanol-0.1% phosphoric acid with a volume ratio of 12.5:16.2:71.3 is used as the mobile phase; column temperature: 30°C; detection wavelength is 340±2nm; Glycoside peak calculation should not be less than 3000;

[0050] (2) Preparation of reference substance solution Take appropriate amount of scutellarein and scutellarein reference substance respectively, accurately weigh them, put them in a brown measuring bottle, add 70% methanol to make each 1ml contains 40 μg of scutellarin and 12 μg of scutellarein The solution, as the reference substance solution;

[0051] (3) Preparation of the test solution Take 0.5g of Scutellaria barbata medicinal material fine powder, accurately weighed; or 0.1g of Scutellaria barbata formula granules, accurately weighed; respectively put in conical flasks with stoppers, and accurately add 70% methanol 25ml, densely packed, weighed, the fine powder of medicinal materi...

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Abstract

The invention relates to a content determination method for scutellarin and scutellarein in sculellaria barbata medicinal material and formula granule of sculellaria barbata medicinal material. The content determination method is characterized in that common isocratic elution is adopted, acetonitrile-methyl alcohol-0.1 percent phosphoric acid of which the volume ratio is 12.5:(15 to 16.2):(71.3 to 72.5) is taken as a mobile phase, and the contents of the scutellarin and the scutellarein in the sculellaria barbata medicinal material and the formula granule of the sculellaria barbata medicinal material are simultaneously determined at a wavelength of 340+ / -2nm. The method is simple, convenient, fast, and easy to popularize and master; the separations of wave crests on three different chromatographic columns are good. Compared with a method of recording under a sculellaria barbata medicinal material item of the first part of the pharmacopeia, the prominent improvement is that a sample treatment process is simplified, the time and cost are saved, the determination is advanced from single component determination of the scutellarin to the simultaneous determination of the two components, the quality controllability is improved, and the defects that impurity peaks exist and the content results are inaccurate are overcome.

Description

technical field [0001] A method for determining the content of scutellarein and scutellarein in Scutellaria barbata or its formula granules. Background technique [0002] Scutellaria barbata is the dried whole herb of Lamiaceae plant Scutellaria barbata D.Don. It is a commonly used traditional Chinese medicine, which has the effects of clearing heat and detoxifying, removing blood stasis and diuresis. For furuncle swelling, sore throat, tumbling pain, edema, jaundice, snake and insect bites. It mainly contains flavonoids, such as baicalein, wogonin, apigenin, luteolin, naringenin, scutellarin, 7-hydroxy-5,8 dimethoxyflavone, 7-hydroxy-5,8 dimethoxy Methoxyflavone-7-0-β-D-glucoside, 5, 7, 8, 2-tetrahydroxyflavone-7-0-β-D-glucoside, etc., also contains some diterpene and triterpene lactones class etc. Its quality standard does not include the TLC identification item under the Chinese Pharmacopoeia 2015 edition of a section of Scutellaria barbata medicinal materials, and co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 牛丽颖田宇柔王鑫国韩桂茹
Owner HEBEI UNIV OF CHINESE MEDICINE
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