A method for embedding riboflavin in peptide nanotubes to modify the anode of microbial fuel cells
A fuel cell and riboflavin technology, applied in biochemical fuel cells, battery electrodes, circuits, etc., can solve problems such as poor conductivity and reduce the electron transfer effect of electronic intermediaries, so as to improve electricity production performance, good conductivity and biological Compatibility, the effect of reducing internal resistance
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Embodiment 1
[0021] (1) Use carbon paper as the electrode material, cut it into a size of 2.5cm×2.5cm, soak the carbon paper in acetone solution for pretreatment, the soaking time is 12h, take it out, rinse it repeatedly with deionized water for 3 times, and place it Dry in an oven at 105°C for 3 hours, then transfer to a muffle furnace for 30 minutes at 370°C to obtain pretreated carbon paper;
[0022] (2) Immerse the pretreated carbon paper in an aqueous solution of polyethyleneimine with a concentration of 3 g / L, immerse it for 10 minutes, and then dry it under nitrogen. Repeat this three times to make the surface of the carbon paper attach positively charged polyethyleneimine layer;
[0023] (3) Mix the phenylalanine dipeptide aqueous solution with a concentration of 4mg / mL and the riboflavin aqueous solution of 10mM according to the volume ratio of 1:1, and the concentration of the mixed phenylalanine dipeptide is 2mg / mL, riboflavin The concentration of the protein was 5 mM. After vo...
Embodiment 2
[0025] (1) Using carbon cloth as the electrode material, soak carbon cloth (5cm×5cm) in acetone solution for 24 hours to remove organic matter on the surface, rinse it with deionized water three times after taking it out, and place it in an oven at 105°C to dry Then move it into a muffle furnace and dry it at 370°C for 30 minutes to obtain a pretreated carbon cloth;
[0026] (2) Immerse the pretreated carbon cloth in a polyethyleneimine solution with a concentration of 5 g / L, immerse it for 10 minutes, and then dry it under nitrogen. Repeat 4 times to make the surface of the carbon cloth attach positively charged poly Ethyleneimine layer;
[0027] (3) Mix the phenylalanine dipeptide aqueous solution with a concentration of 9mg / mL and the riboflavin aqueous solution of 9mM according to the volume ratio of 1:2, the concentration of the mixed phenylalanine dipeptide is 3mg / mL, and the riboflavin The concentration of PNTs on the electrode is 6mM, take 200μL of the mixed solution ...
Embodiment 3
[0029] (1) Using carbon cloth as the electrode material, soak carbon cloth (5cm×5cm) in acetone solution for 24 hours to remove organic matter on the surface, rinse it with deionized water three times after taking it out, and place it in an oven at 105°C to dry Then move it into a muffle furnace and dry it at 370°C for 30 minutes to obtain a pretreated carbon cloth;
[0030] (2) Immerse the pretreated carbon cloth in a polyethyleneimine solution with a concentration of 5 g / L, immerse it for 10 minutes, and then dry it under nitrogen. Repeat 4 times to make the surface of the carbon cloth attach positively charged poly Ethyleneimine layer;
[0031](3) Mix the phenylalanine dipeptide aqueous solution with a concentration of 6mg / mL and the riboflavin aqueous solution of 6mM according to the volume ratio of 1:2, the concentration of the mixed phenylalanine dipeptide is 3mg / mL, and the riboflavin The concentration of the carbon cloth is 3mM, soak the carbon cloth in the mixture of...
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