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New uses for fluidic devices

A fluid, the first fluid technology, applied in biochemical cleaning equipment, enzymology/microbiology equipment, specific-purpose bioreactors/fermenters, etc.

Active Publication Date: 2019-05-28
GRADIENTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The limitation of the droplet system is that only a single antibiotic concentration can be tested

Method used

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  • New uses for fluidic devices
  • New uses for fluidic devices
  • New uses for fluidic devices

Examples

Experimental program
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Embodiment Construction

[0040] Throughout the drawings, the same reference numerals are used for similar or corresponding elements.

[0041] Embodiments of the present invention relate generally to fluidic devices, and in particular to the use of such fluidic devices in determining the response of microorganisms to a test substance. It has been concluded that fluidic devices such as microfluidic devices can be designed to be particularly suitable for monitoring or measuring the response of microorganisms to various test substances. In more detail, such fluidic devices provide an efficient tool for rapidly determining the response of microorganisms to a test substance, or indeed to any combination of multiple test substances, with a culture medium containing a three-dimensional (3D) culture substrate. chamber, the three-dimensional culture substrate comprising a culture of associated microorganisms.

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Abstract

The present invention discloses a fluidic device (1) having a culture chamber (10) configured to accommodate a 3D culture substrate (2) comprising a culture of microorganisms (6). Concentration gradients of the test substances are established on the 3D culture substrate ( 2 ) by providing corresponding fluid flows at different end portions ( 12 , 14 ) of the culture chamber ( 10 ) and containing different concentrations of the test substances . The response of the microorganism (6) to the test substance is determined based on the position of the border zone (5) in the 3D culture matrix (2).

Description

technical field [0001] Embodiments relate generally to fluidic devices, and in particular to the use of such fluidic devices for determining the response of microorganisms to a test substance. Background of the invention [0002] Antibiotic-resistant bacteria are becoming a global problem because antibiotics cannot kill them or stop them from dividing. The passage time of bacteria can in many cases be very fast (approximately 20 minutes), and due to the short passage time and relative genetic instability of bacteria, bacteria can quickly acquire resistance to antibiotics. The increasing prevalence of antibiotic-resistant bacterial infections, some of which have become multi-resistant, sometimes means that there are no effective antibiotics available to stop the growth of these bacteria. These multi-resistant bacteria are a serious public health problem because patients infected with them can die because their bacterial infection cannot be treated. [0003] Traditional meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04B01L3/00C12M1/00
CPCB01L3/5027C12M23/16C12M25/14C12M1/16C12Q1/02C12M21/00C12M41/46C12Q1/18
Inventor J·克雷于格S·索斯朗德吴志刚
Owner GRADIENTECH
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