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A high-efficiency degradation bacterium s1 of lignocellulosic substances and its application

A lignocellulosic and high-efficiency degrading bacteria technology, which is applied to the treatment of bacteria, microorganisms, and biological organic parts, can solve the problems of low enzyme activity and achieve the effects of enzyme production, stable enzyme activity, rapid proliferation, and wide application

Active Publication Date: 2018-11-23
北京中农国泰科技有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, the use of fungi to degrade lignocellulose generally has the problem of low enzyme activity

Method used

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  • A high-efficiency degradation bacterium s1 of lignocellulosic substances and its application
  • A high-efficiency degradation bacterium s1 of lignocellulosic substances and its application
  • A high-efficiency degradation bacterium s1 of lignocellulosic substances and its application

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specific Embodiment approach 1

[0024] Specific embodiment 1: A lignocellulosic material efficient degrading bacterium S1 of the present embodiment, it is Enterobacter hormaechei (Enterobacter hormaechei) S1, preserved in the general microorganism center of China Microorganism Culture Collection Management Committee, and the preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the city. The preservation date is December 12, 2014, and the preservation number is CGMCC No.10165.

[0025] Enterobacter hormaechei (Enterobacter hormaechei) S1 of the present embodiment is a gram-negative bacterium, and the bacterial cell shape of the strain is rod-shaped, and the cell size is 0.55-0.58×1.4-1.5 μm, does not form spores, and has no flagella and pods Membrane; forms round, opaque, milky-white colonies with smooth protrusions and neat edges on LB medium (such as figure 1 shown). The results of the physiological and biochemical reactions of the strain included 18 items and 28 in...

specific Embodiment approach 2

[0094] Embodiment 2: The application of a lignocellulosic substance-degrading bacteria S1 in this embodiment is used for degrading lignocellulosic substances.

[0095] Bacterial strain of the present invention is carried out following functional detection:

[0096] Enterobacter hormaechei (Enterobacter hormaechei) S1 of the specific embodiment was tested for weight loss rate and lignin, cellulose and hemicellulose degradation of bagged fungus waste and corn stalks to verify its unique functions. details as follows:

[0097] 1. Determination of weight loss rate of bagged fungus waste and corn stalks

[0098] 1.1 Corn stalk powder

[0099] Corn stalks were taken from the experimental field of the laboratory in Hulan Campus, Heilongjiang University, Harbin City, Heilongjiang Province in October 2012, dried, crushed and passed through a 40-mesh sieve, and set aside.

[0100] 1.2 bags of fungus waste

[0101] Bags of fungus waste were provided by Mudanjiang Branch of Heilongjia...

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Abstract

The invention relates to a lignocellulos substance high-efficiency degrading bacterium S1 and application thereof. The lignocellulos substance high-efficiency degrading bacterium S1 is Enterobacter hormaechei S1 and was preserved with preservation number CGMCC No.10165 on December 12, 2014 by China General Microbiological Culture Collection Center. The bacterium is used for degrading lignocellulos substances. The strain provided by the invention has the advantages of quick proliferation, strong applicability and wide application; a weight-loss ratio of bagged agaric wastes after being fermented by Enterobacter hormaechei S1 is above 31 percent and a weight-loss ratio of corn stalks after being fermented by Enterobacter hormaechei S1 is above 33 percent.

Description

technical field [0001] The invention relates to a high-efficiency degradation bacterium S1 of lignocellulosic substances and its application. Background technique [0002] my country is a large agricultural country, and the amount of agricultural waste generated is extremely huge. Due to the limitations of economic benefits and technology popularization, most of the agricultural waste is extensively and inefficiently used and the state of idleness is serious, resulting in waste of resources and environmental pollution. Waste has become China's largest source of pollution. However, there is a large amount of lignocellulose in agricultural waste, which needs to be developed and utilized urgently. [0003] Lignin is the second most abundant organic renewable resource after cellulose in nature, and it is also one of the most difficult components for microorganisms to degrade. In recent years, some lignin-degrading fungi have begun to be applied in practice, but further developm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCY02W30/40
Inventor 杨峰山刘春光孙永帅吴奇丁仕波杨微王鲲鹏王庆华魏才强曲金玲张慧于文全
Owner 北京中农国泰科技有限公司
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