A kind of induction method of eel algae callus

A technology of callus and eelgrass, applied in the field of plant tissue culture research, can solve problems such as patents and literatures that have not been reported, and achieve the effect of easy surface disinfection and short induction time

Inactive Publication Date: 2017-11-14
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the present invention, the embryo of Z. echinopsis is used as the explant to induce the callus in a short period of time. At present, there are no reports in the relevant patents and literatures using this technical solution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: a kind of induction method of eelgrass callus, its implementation step comprises:

[0018] 1. The selection of explants: when the fruit of Zelpium nodosum is almost ripe, collect its healthy spathe inflorescences, peel off the bract leaves outside the spathe inflorescences, leave only the inflorescences and the fruits on them, and wash them Soak and disinfect with 15% hydrogen peroxide in the ultra-clean workbench for 20 minutes, then rinse with sterilized sea water 3-5 times to obtain sterile inflorescences, remove the fruits on the inflorescences one by one, cut open the pericarp, and take out the sterile embryos;

[0019] 2. Induction culture of callus: Inoculate the sterile embryos in the induction medium under sterile conditions, and culture them at 18°C ​​in the dark. When culturing for nearly 30 days, the cotyledon can be seen to radiate, and the cotyledon can be seen to swell with the radiance of the cotyledon, and the callus can be formed after ab...

Embodiment 2

[0020] Embodiment 2: a kind of induction method of eosinopsis somatic embryo, its implementation steps are:

[0021] 1. Selection of explants: take mature seeds as explants, select mature eelgrass seeds with plump appearance and brown seed coat, rinse them with sea water, and soak them in 15% hydrogen peroxide in an ultra-clean workbench After 20 minutes, rinse with sterilized seawater for 3-5 times, dry the water with sterile filter paper, cut the seed coat with a scalpel, and take sterile embryos;

[0022] 2. Induction culture of somatic embryos: inoculate the sterile embryos in the induction medium under sterile conditions, and culture them at 15°C in the dark. When cultured to 20 days, it can be seen that the tissue of the lower part of the embryo expands, and at the same time, the cotyledon emerges. With the emergence of the cotyledon, it can be seen that the place where the cotyledon emerges also begins to expand. After about 50 days, the formation of callus can be seen ...

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PUM

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Abstract

The present invention relates to a method for inducing callus of Zarphyria spp., which uses the embryos of C. argentina as explants, takes out sterile embryos from seeds or fruits that have been sterilized on the surface, and inoculates them into a callus induction medium Above, induction culture was carried out at 15-25°C and dark conditions, and transplanted every half a month. After culturing for nearly 20-30 days, the cotyledon can be seen to swell, and the cotyledon can be seen to swell along with the cotyledon. After about 50 days, the callus is formed. The induction medium is a solid medium with MS, 1 / 2MS medium as the base medium, and supplemented with organic supplements, carbon source, auxin and sea salt. The method of the present invention can quickly and effectively induce the callus of Zinaria spp., which provides important technical support for the regeneration of aseptic tissue-cultured seedlings of Z. Foundation.

Description

technical field [0001] The invention belongs to the field of plant tissue culture research, and the invention relates to a method for inducing callus of Z. Background technique [0002] Eelweed ( Zostera marina L.) is a representative species of seagrass in the world, and it is also the dominant species of seagrass in the sediment sea area of ​​northern my country. The seagrass field constructed by it has extremely important ecological service functions: it provides habitat, breeding and hiding places for many economic fish and other marine organisms; its numerous humus provides excellent natural bait for plankton, young fish and shrimp and other marine animals ; It can absorb marine pollutants, play a role in purifying sea water quality and improving the transparency of sea water; it can resist wind, waves and tides, and is a natural barrier to protect the coast. [0003] However, in the past century, due to various human factors, the population of eelweed has declined on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郑凤英陈玉刘雪芹金艳梅张伟李乐乐
Owner SHANDONG UNIV
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