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A technology of callus and eelgrass, applied in the field of plant tissue culture research, can solve problems such as patents and literatures that have not been reported, and achieve the effect of easy surface disinfection and short induction time
Inactive Publication Date: 2016-03-16
SHANDONG UNIV
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Problems solved by technology
[0005] In the present invention, the embryo of Z. echinopsis is used as the explant to induce the callus in a short period of time. At present, there are no reports in the relevant patents and literatures using this technical solution.
Method used
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Embodiment 1
[0018] Embodiment 1: a kind of induction method of eelgrass callus, its implementation step comprises:
[0019] 1. The selection of explants: when the fruit of Zelpium nodosum is almost ripe, collect its healthy spathe inflorescences, peel off the bract leaves outside the spathe inflorescences, leave only the inflorescences and the fruits on them, and wash them Soak and disinfect with 15% hydrogen peroxide in the ultra-clean workbench for 20 minutes, then rinse with sterilized sea water 3-5 times to obtain sterile inflorescences, remove the fruits on the inflorescences one by one, cut open the pericarp, and take out the sterile embryos;
[0020] 2. Induction culture of callus: Inoculate the sterile embryos in the induction medium under sterile conditions, and culture them at 18°C in the dark. When culturing for nearly 30 days, the cotyledon can be seen to radiate, and the cotyledon can be seen to swell with the radiance of the cotyledon, and the callus can be formed after ...
Embodiment 2
[0021] Embodiment 2: a kind of induction method of eosinopsis somatic embryo, its implementation steps are:
[0022] 1. Selection of explants: take mature seeds as explants, select mature eelgrass seeds with plump appearance and brown seed coat, rinse them with sea water, and soak them in 15% hydrogen peroxide in an ultra-clean workbench After 20 minutes, rinse with sterilized seawater for 3-5 times, dry the water with sterile filter paper, cut the seed coat with a scalpel, and take sterile embryos;
[0023] 2. Induction culture of somatic embryos: inoculate the sterile embryos in the induction medium under sterile conditions, and culture them at 15°C in the dark. When cultured to 20 days, it can be seen that the tissue of the lower part of the embryo expands, and at the same time, the cotyledon emerges. With the emergence of the cotyledon, it can be seen that the place where the cotyledon emerges also begins to expand. After about 50 days, the formation of callus can be see...
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Abstract
The present invention relates to a zostera marina callus induction method, which comprises that the embryo of zostera marina is adopted as an explant, the sterile embryo is taken out from surface sterilized seeds or fruits, the obtained embryo is inoculated into a callus induction culture medium, induction culture is performed at a temperature 15-25 DEG C under a dark condition, transfer inoculation is performed every two weeks, cotyledon is sprouted when the culture is performed for nearly 20-30 d, the cotyledon sprouting position can expand along with the cotyledon sprouting, and the callus is formed after about 50 d, wherein the induction culture medium is a solid culture medium adopting a MS culture medium and a 1 / 2MS culture medium as a base culture medium and added with an organic additive, a carbon source, auxin and sea salt. With the method of the present invention, the zostera marina callus can be quickly and efficiently induced, the important technical support is provided for the the zostera marina sterile tissue culture seedling regeneration and the tissue culture rapid propagation system establishing, and the foundation is established for the successful seaweed tissue culture.
Description
technical field [0001] The invention belongs to the field of plant tissue culture research, and the invention relates to a method for inducing callus of Z. Background technique [0002] Eelweed ( Zostera marina L.) is a representative species of seagrass in the world, and it is also the dominant species of seagrass in the sediment sea area of northern my country. The seagrass field constructed by it has extremely important ecological service functions: it provides habitat, breeding and hiding places for many economic fish and other marine organisms; its numerous humus provides excellent natural bait for plankton, young fish and shrimp and other marine animals ; It can absorb marine pollutants, play a role in purifying sea water quality and improving the transparency of sea water; it can resist wind, waves and tides, and is a natural barrier to protect the coast. [0003] However, in the past century, due to various human factors, the population of eelweed has declined on...
Claims
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Application Information
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