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Chick embryo bioactive peptide, preparation method and applications

A chicken embryo active peptide and chicken embryo technology, which is applied in the biological field to achieve the effects of active protection, easy operation and improved extraction efficiency

Active Publication Date: 2016-03-23
耿威
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few studies on peptides in chicken embryos, and further development and utilization should be carried out in the research of cosmetics, health products and medicines.

Method used

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  • Chick embryo bioactive peptide, preparation method and applications
  • Chick embryo bioactive peptide, preparation method and applications
  • Chick embryo bioactive peptide, preparation method and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Hatching chicken embryos: Take SPF grade fertilized eggs and put them into the incubator for incubation. The incubation temperature is controlled at 37.5°C, the relative humidity is 60%, and the eggs are automatically turned every 2 hours. After hatching to 14 days, take out the fertilized eggs , put it in a freezer at -20°C, stop the incubation and save it for later use.

[0055] (2) Homogenization and cell crushing: surface disinfection of the hatched eggs, removal of egg shells, taking out the chicken embryos, homogenizing the chicken embryos with a tissue homogenizer at 4°C, pouring them into a sterilized sealed box, Put it in a freezer at -30°C for 72 hours, and after thawing, perform cell ultrasonic disruption in an environment of 4°C, add an equal amount of water for injection, and place it in a freezer at 4°C for use.

[0056] (3) Enzymatic hydrolysis and centrifugation: Take the refrigerated solution after cell crushing, heat it in a water bath to 50°C, adj...

Embodiment 2

[0059] (1) Hatching chicken embryos: Take SPF grade fertilized eggs and put them in the incubator for incubation. The incubation temperature is controlled at 37.5°C and the relative humidity is 60%. The eggs are automatically turned every 2 hours. After hatching to 17 days, take out the fertilized eggs , put it in a freezer at -20°C, stop the incubation and save it for later use.

[0060] (2) Homogenization and cell crushing: surface sterilize the hatched eggs, remove the eggshells and take out the chicken embryos, homogenize the chicken embryos with a tissue homogenizer at 5°C, and pour them into a sterilized sealed box. Freeze in a freezer at -20°C for 80 hours, dissolve and ultrasonically disrupt the cells in an environment at 8°C, add an equal amount of water for injection, and place it in a freezer at 8°C for use.

[0061] (3) Enzymatic hydrolysis and centrifugation: take the refrigerated liquid after the cells are broken, heat it in a water bath to 55°C, adjust the pH to...

Embodiment 3

[0064] (1) Hatching chicken embryos: Take SPF grade fertilized eggs and put them in the incubator for incubation. The incubation temperature is controlled at 37.5°C and the relative humidity is 60%. The eggs are automatically turned every 2 hours. After hatching to 19 days, take out the fertilized eggs , put it in a cold storage at 0°C, stop the incubation and save it for later use.

[0065] (2) Homogenization and cell crushing: surface disinfection of hatched eggs, removal of egg shells, taking out chicken embryos, homogenizing chicken embryos with a tissue homogenizer at 0°C, pouring them into a sterilized sealed box, Put it in a freezer at -40°C for 60 hours, and after thawing, perform cell ultrasonic disruption in an environment of 0°C, add an equal amount of water for injection, and place it in a freezer at 0°C for use.

[0066] (3) Enzymatic hydrolysis and centrifugation: take the frozen frozen solution after cell crushing, heat it in a water bath to 45°C, adjust the pH ...

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Abstract

The invention relates to the biotechnology field, and especially relates to a chick embryo bioactive peptide, a preparation method and applications. The provided chick embryo bioactive peptide comprises a chick embryo bioactive peptide and free amino acids, wherein the molecular weight of the chick embryo bioactive peptide is less than 10kDa. The provided chick embryo bioactive peptide can protect activity of human fibroblasts. When the experimental group with addition of the provided chick embryo bioactive peptide is compared with a control group without addition of the provided chick embryo bioactive peptide, activity of human fibroblasts in the experimental group is protected. When an experiment group is compared with a control group without ultraviolet radiation, activity of human fibroblasts of the experiment group is the same as and even slight higher than activity of human fibroblasts of the control group. The preparation method for the chick embryo bioactive peptide is simple in operation, through homogenization, freeze thawing and ultrasonic fragmentation, cell membranes are crushed furthest, thus the bioactive peptide in the cells is dissolved out well, biotechnology extraction processes of centrifugation, ultrafiltration and the like are carried out, and therefore the extraction efficiency is raised.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a chicken embryo active peptide and its preparation method and application. Background technique [0002] Amino acid is a general term for a class of organic compounds containing amino and carboxyl groups. Among them, glycine (GLY) has the nutritional effect of improving muscle vitality and nourishing the human body; leucine (LEU) has the effect of promoting skin and wound healing; methionine (MET) has the function of removing fat and can promote the synthesis of skin protein ; Tyrosine (TYR) has the effect of treating neuroinflammation and participates in the repair of skin inflammation. It is related to the formation of pigment, and it will benefit albinism when it is lacking; histidine (HIS) has a repairing effect on allergic skin; threonine (THR) is necessary for human body, and it will make people lose weight when it is lacking; alanine (ALA) can Promote the metabolism (decomp...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/98A61Q17/04A61Q19/00
CPCA61K8/64A61K8/982A61Q17/04A61Q19/004
Inventor 耿威金英花战铁楠李杨耿晓宇王莉红王笑琳耿利
Owner 耿威
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