Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of animal and human primitive pluripotent stem cell, kit and use thereof

A technology of pluripotent stem cells and animals, which is applied in the field of preparing primitive pluripotent stem cells of animals and humans, and can solve problems such as troubles and great differences in conditions

Active Publication Date: 2016-03-30
PEKING UNIV +1
View PDF4 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the small-molecule conditions used in these studies are quite different from those used on mouse endocyst-like pluripotent stem cells, which also brings new challenges to the search for conditions for establishing in vitro endocytic-like pluripotent stem cells from other animal species. Obsession (GafniO et al., 2013, ChanYSetal., 2013 and WareCBetal., 2013)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of animal and human primitive pluripotent stem cell, kit and use thereof
  • Preparation method of animal and human primitive pluripotent stem cell, kit and use thereof
  • Preparation method of animal and human primitive pluripotent stem cell, kit and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0141] In the following, the embodiment of the present invention will be specifically described by taking inner cell mass-like pluripotent stem cells as an example of primitive pluripotent stem cells. It should be pointed out that the following specific experimental schemes are only for the purpose of illustration, not specifically limiting the scope of the present invention.

[0142] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0143] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0144] (1) Isolation and culture of primary rhesus monkey adult fibroblasts

[0145] Mainly used reagents:

[0146] DMEM-HG+10%FBS (common)+1% double antibody (penic / streptomycin)

[0147] 5mg / ml DispaseII: Dissolve 0.5g DispaseII powder in 100ml PBS, filter and sterilize.

[0148] 0.25% trypsin

[0149] PBS buffer

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
centrifugal forceaaaaaaaaaa
centrifugal forceaaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method of an animal and human primitive pluripotent stem cell, a kit and use thereof. The preparation method of the animal and human primitive pluripotent stem cell provided by the invention includes letting an animal and human primitive ectoderm-like pluripotent stem cell contact at least one MAPK pathway inhibiting factor, at least one WNT signaling pathway regulatory factor, at least one LIF / STAT3 pathway regulatory factor and at least one FGF pathway regulatory factor so as to generate the animal and human primitive pluripotent stem cell. The invention also relates to a composition, medium or kit for preparation of the animal and human primitive pluripotent stem cell, as well as the primitive pluripotent stem cell prepared thereby and its use. The primitive pluripotent stem cell involved in the invention can be used for preparation of the chimeras of different animal and human species and the like, and is expected to be used for disease model establishment, drug testing and organ transplantation, etc.

Description

technical field [0001] The invention relates to a method for preparing animal and human primitive pluripotent stem cells, a kit and application thereof. Background technique [0002] (1) Two pluripotent states of pluripotent stem cells: [0003] Studies in rodents such as mice and rats have shown that pluripotent stem cells can be divided into two distinct pluripotent states: mouse embryonic stem cells established from the inner cell mass of pre-implantation mouse embryos The inner cell mass-like pluripotent stem cells are represented by the inner cell mass-like pluripotent stem cells and the primitive ectoderm-like pluripotent stem cells are represented by the mouse primitive ectoderm stem cells established from the primitive ectoderm of post-implantation mouse embryos. These two types of pluripotent stem cells differ considerably in their gene expression profiles, maintenance of required signaling pathways, and ability to chimerize. For example, inner cell mass-like plur...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0735C12N5/10A01K67/027
CPCC12N2501/115C12N2501/727C12N2501/734C12N2506/1307C12N5/0696A61P43/00A01K67/027C12N15/8509A01K2227/105A01K2267/025A01K2207/12C12N2501/20C12N2501/50C12N2506/09
Inventor 邓宏魁赵扬方日国刘康杨炜峰
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com