Preparation method of animal and human primitive pluripotent stem cell, kit and use thereof

Abstract

The invention relates to a preparation method of an animal and human primitive pluripotent stem cell, a kit and use thereof. The preparation method of the animal and human primitive pluripotent stem cell provided by the invention includes letting an animal and human primitive ectoderm-like pluripotent stem cell contact at least one MAPK pathway inhibiting factor, at least one WNT signaling pathway regulatory factor, at least one LIF / STAT3 pathway regulatory factor and at least one FGF pathway regulatory factor so as to generate the animal and human primitive pluripotent stem cell. The invention also relates to a composition, medium or kit for preparation of the animal and human primitive pluripotent stem cell, as well as the primitive pluripotent stem cell prepared thereby and its use. The primitive pluripotent stem cell involved in the invention can be used for preparation of the chimeras of different animal and human species and the like, and is expected to be used for disease model establishment, drug testing and organ transplantation, etc.

Description

technical field [0001] The invention relates to a method for preparing animal and human primitive pluripotent stem cells, a kit and application thereof. Background technique [0002] (1) Two pluripotent states of pluripotent stem cells: [0003] Studies in rodents such as mice and rats have shown that pluripotent stem cells can be divided into two distinct pluripotent states: mouse embryonic stem cells established from the inner cell mass of pre-implantation mouse embryos The inner cell mass-like pluripotent stem cells are represented by the inner cell mass-like pluripotent stem cells and the primitive ectoderm-like pluripotent stem cells are represented by the mouse primitive ectoderm stem cells established from the primitive ectoderm of post-implantation mouse embryos. These two types of pluripotent stem cells differ considerably in their gene expression profiles, maintenance of required signaling pathways, and ability to chimerize. For example, inner cell mass-like plur...

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Problems solved by technology

However, the small-molecule conditions used in these studies are quite different from those used on mouse endocyst-like pluripotent stem cells, which also brings new challenges to the search for conditions for establishing in vitro endocytic-like pluripotent stem cells from other animal species. Obsession (GafniO et al., 2013, ChanYSetal., 2013 and WareCBetal., 2013)

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