Tissue culture detoxification and rapid propagation method of bamboo tree
A technology of tissue culture and bamboo joints, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of unreported seedling raising technology, and achieve the effect of good seedling commodity, high economic benefit, and uniform offspring
Inactive Publication Date: 2018-04-17
佛山市农业科学研究所
View PDF5 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Seedling raising technology in bamboo tree vegetative propagation has not been reported yet
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0035] 1. Operation process
[0036] see figure 1 , in the embodiment of the present invention, the tissue culture detoxification rapid propagation method of bamboo tree,
[0037] 1. Medium preparation:
[0038] 1-1. Preparation of mother liquor: The composition and ratio of MS medium are well known to those skilled in the art, including potassium nitrate, calcium chloride, vitamins and other 19 ingredients, and additional BA, NAA and IBA are also well-known products in the art , are prepared in strict accordance with the requirements of the instructions.
[0039] 1-2. Preparation of culture medium: According to the magnification of the ratio of the mother liquor, combined with the quantity of the culture medium to be prepared, measure the corresponding amount of mother liquor; after the distilled aqueous solution containing the corresponding amount of agar powder and sugar is heated and melted, Add the taken mother liquor into it, stir evenly after settling, adjust the pH ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a tissue culture virus elimination and rapid propagation method of Carallia brachiata. The method comprises the processes of Carallia brachiata material pretreatment, Carallia brachiata material selection and treatment, disinfection, virus and pollution elimination, rooting culture and greenhouse domestication. An initial culture medium, a shoot tip virus elimination culture medium, a subculture medium and a rooting culture medium are respectively used in the virus and pollution elimination process to culture the Carallia brachiata material, and an improved MS culture medium containing macroelements with the NH4NO3 concentration of 1500mg / L and 6g / L of agar powder and having a pH value of 5.8-6.0 is used to realize virus elimination and rapid propagation, so the speed is fast, the propagation coefficient is large, the subculture cycle is about 40d, the rooting cycle is about 25d, and virus-free seedlings can be obtained; and an asexual propagation technology is adopted, so progenies are consistent, the excellent properties of a female parent are well maintained, and the economic benefit is high.
Description
technical field [0001] The invention relates to the field of plant detoxification and rapid propagation, in particular to a method for detoxification and rapid propagation of bamboo tree tissue culture. Background technique [0002] Bamboo tree belongs to the mangrove family, which is a tall evergreen tree species. It is also called annatto. It is tall, straight, and the wood is firm, shiny, fine-grained, and easy to construct. It is a good material for furniture, decorative carvings, and small buildings. ; Because of its dense leaves, many germinations, drought tolerance and barrenness, it can not only survive under severe pollution conditions, but also adapt to harsh soil environments and achieve the purpose of purifying the environment. air pollutants, especially SO 2 , the resistance level of FH gas reaches the first level, so the bamboo tree is known as the "Green King Kong". It is an ideal new garden ornamental and good anti-pollution precious tree species. Secondary...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 梁普兴韩向峰李志芳冯伟明李强李湘妮
Owner 佛山市农业科学研究所