Kit for quantitative detection of anti-Jo-1 antibody IgG by using magnetic particle chemiluminescence, and preparation method and detection method thereof
A chemiluminescent, quantitative detection technology, applied in the field of immunological detection, can solve problems such as poor repeatability, long reaction time, and narrow linear range
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Embodiment 1
[0066] A kit for the quantitative detection of anti-Jo-1 antibody IgG using magnetic particle chemiluminescence. The kit includes: Anti-Jo-1IgG calibrator, Anti-Jo-1IgG reagent No. 1, Anti-Jo-1IgG reagent No. 2, Anti-Jo-1IgG magnetic separation reagent, Anti-Jo-1IgG quality control material and cleaning solution.
[0067] Evaluation of analytical performance of the kit of the present invention:
[0068] 1) Accuracy evaluation
[0069] Add the anti-Jo-1 antibody IgG sample A with a concentration of about 200RU / mL and allow its concentration deviation of ±20% to the serum or other corresponding matrix sample B. The volume of A added does not exceed the total volume of A+B 10%, the recovery rate R is calculated according to formula (1), the recovery rate of this method is in the range of 85-115%, see Table 1 for data.
[0070] R = C × ( V 0 + V ) - C 0 × V 0 V × C S × 100 % ... ( 1 )
[0071] R: recovery rate;
[...
Embodiment 2
[0112] A method for preparing a kit for quantitatively detecting anti-Jo-1 antibody IgG using magnetic particle chemiluminescence, including the following steps:
[0113] Step 1. The preparation of the Anti-Jo-1IgG calibrator includes: Anti-Jo-1IgG calibrator diluent preparation steps and Anti-Jo-1IgG calibrator preparation steps:
[0114] The preparation steps of the Anti-Jo-1IgG calibrator diluent include:
[0115] Add 800mL purified water and 11.2g of Tris to the container, stir and mix well, then add 8.5g of sodium chloride and 2mL of Proclin300, stir until completely dissolved, adjust the pH to 7.0-7.5, add 40g of bovine serum white Put the protein into the container, stir until it is completely dissolved, then adjust the pH to 7.0-7.5, dilute to 1L with purified water, filter with a 0.2μm filter, and store the obtained calibrator dilution at 2-8℃ for later use;
[0116] The preparation steps of the Anti-Jo-1IgG calibrator include:
[0117] Prepare the anti-Jo-1 antibody IgG to 0...
Embodiment 3
[0134] On the basis of Example 2, the kit further includes a cleaning solution, and the preparation steps of the cleaning solution:
[0135] Add 800mL purified water, Tris12.1g and NaCl8.5g to a 1L container, stir well until completely dissolved; then add 5g Tween-20 and 5g Triton100, stir well until completely mixed, adjust the pH value to 7.5 -8.0, a constant volume of 1L, filter with a 0.2μm filter, store at a temperature of 2-8°C, dilute and mix the cleaning solution at a ratio of 7.5mL to 100mL with purified water before use.
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