Cell culture container and container in which cell culture has been accommodated

Abstract

An inner pouch (20) of a cell culture container (10) is encapsulated in an outer pouch (50) in a state wherein rubber stoppers (24) are exposed to the outside. A closed space (56) surrounded by the outside surface of the inner pouch (20) and the inside surface of the outer pouch (50) is in a sterilized state. Therefore, a sterilized state can be maintained for the outside surface of the inner pouch (20) for accommodating variable material containing cells. In addition, a sterilization process can be performed prior to accommodating variable material containing cells and therefore settings for conditions for the sterilization process can be carried out comparatively easily without considering effects thereof on the variable material containing cells. Furthermore, the bottom surfaces and side surfaces of recessed parts in holding parts (30) and the bottom surfaces and side surfaces of covers allow liquid to pass through; therefore, if the variable material containing cells is held in a holding space (30a) and then a liquid medium is added to this inner pouch (20) and the variable material containing cells is cultured, cells are propagated substantially uniformly in the entire variable material containing cells.

Description

technical field [0001] The present invention relates to a cell culture container and a cell culture storage completion container. Background technique [0002] Conventionally, there are known containers for cell-containing substances for culturing or accommodating cell-containing substances such as tissue pieces and cell suspensions. For example, Patent Document 1 discloses a cell-containing container including a bag-shaped chamber, an opening and closing portion, and a tissue piece holding portion, the opening and closing portion being used for carrying the tissue piece into the chamber and for transferring the tissue piece from the chamber. It is carried out and opened and closed in the chamber, and the tissue piece holding part holds the tissue piece in the chamber. [0003] Patent Document 1: Japanese Patent Laid-Open No. 2005-87029. [0004] However, in the sterilization of medical equipment, etc., the sterility assurance level (Sterility Assurance level: SAL) is used...

AI Technical Summary

Problems solved by technology

In addition, since the sterilization treatment in the state containing the cell-containing material has the possibility of affecting the cell-containing material due to subtle changes in the processing conditions, the setting of the conditions is complicated and difficult.

Furthermore, since the cell-containing container of Patent Document 1 is aimed at clamping tissue pieces, the variable cell-containing material in a state where the shape is easily deformed by an external force (pressing force based on clamping in Patent Document 1) (containing cytosol or containing cell gel, etc.), it is difficult to maintain the desired shape, and it is not suitable for cell culture to obtain a three-dimensional structure (three-dimensional structure)

In addition, in Patent Document 1, since a cell-containing substance is cultured on a sheet-like culture surface, the portion in contact with the culture surface receives less nutrient supply from the culture medium, making it difficult for cells to proliferate.

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