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A strain with heterotrophic nitrification and aerobic denitrification functions and its application

An aerobic denitrification and heterotrophic nitrification technology, applied in the field of microorganisms, can solve the problem of lack of heterotrophic nitrifying bacteria that can efficiently remove high-concentration ammonia nitrogen, and achieve good application value, simple method and good effect.

Inactive Publication Date: 2019-07-23
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of heterotrophic nitrifying bacteria that can efficiently remove high-concentration ammonia nitrogen. Therefore, screening microorganisms that can efficiently remove high-concentration ammonia nitrogen is still an important research content of heterotrophic nitrifying microorganisms. microbiological reports

Method used

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  • A strain with heterotrophic nitrification and aerobic denitrification functions and its application
  • A strain with heterotrophic nitrification and aerobic denitrification functions and its application
  • A strain with heterotrophic nitrification and aerobic denitrification functions and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the screening and identification of efficiently removing ammonia-nitrogen bacteria

[0039] Efficient removal of ammonia-nitrogen bacteria Screened from activated sludge and landfill leachate, each isolated strain was placed in a 250ml wide-mouth Erlenmeyer flask containing 100ml of nitrification medium, and placed in a shaker at 30°C and 150rpm for 36 hours , take out the culture solution and inoculate it into LB medium, culture it with shaking at 30°C until the bacterial concentration is OD600=2.0, then insert 1% of the inoculum into the nitrification of high ammonia nitrogen wastewater containing ammonia nitrogen concentration less than 2000ppm to be treated In the medium, maintain the dissolved oxygen above 2.0ppm. After 3 days, the waste water was continuously taken and centrifuged to measure the ammonia nitrogen concentration in the supernatant, and the strain with the highest ammonia nitrogen removal efficiency was obtained.

[0040] The method for...

Embodiment 2

[0045] Embodiment 2, application Zobelella DN-7 process ammonia nitrogen-containing wastewater

[0046] The method for treating ammonia nitrogen-containing wastewater by Zobelella DN-7, the specific steps are as follows:

[0047] Inoculate the Zobelella DN-7 strain into the LB medium, and culture it with shaking at a temperature of 30°C until the bacterial concentration is OD600=2.0, then insert the 1% inoculum containing ammonia nitrogen concentration less than In the nitrification medium of 2000ppm high ammonia nitrogen wastewater, maintain the dissolved oxygen above 2.0ppm. After 3 days, the wastewater was continuously taken and centrifuged to measure the ammonia nitrogen concentration in the supernatant.

[0048] The results show that the Zobelella DN-7 obtained by the invention has obvious removal effects on ammonia nitrogen and total nitrogen, and there is no increase in nitrite nitrogen and nitrate nitrogen. The invention has simple process, can tolerate and quickly d...

Embodiment 3

[0049] Embodiment 3, Zobelella DN-7 removes the influence factor of ammonia nitrogen

[0050] 1. The effect of carbon source on the removal of ammonia nitrogen by Zobelella DN-7

[0051] Each selects methanol, ethanol, glucose, sucrose, sodium acetate, sodium succinate, and sodium citrate as the sole carbon source of the nitration medium, and the quality of each carbon source makes C / N=12 in the medium, and the Other ingredients are: (NH 4 ) 2 SO 4 , 1.0g; K 2 HPO 4 , 5.0g; KH 2 PO 4 , 2.0g; MgSO 4 ·7H 2 O, 0.2g; trace element solution, 2mL; distilled water 1L, pH=8.0. Inoculate the Zobelella DN-7 into the nitrification medium containing the ammonia nitrogen concentration to be treated less than 2000ppm high ammonia nitrogen wastewater, after shaking and culturing for 36 hours, measure and calculate according to the implementation case 1, the results are as follows figure 1 shown. The results show that the ammonia nitrogen removal rate of Zobelella DN-7 using sucros...

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Abstract

The invention discloses a strain with heterotrophic nitrification and aerobic denitrification functions and an application of the strain. The strain is Zobellella taiwanensis DN-7, is preserved in CCTCC (China Center for Type Culture Collection) with the CCTCC NO: M2015648 and can tolerate ammonia-nitrogen wastewater with the concentration higher than 2,000 ppm. The invention further discloses a method for treating ammonia-nitrogen wastewater by utilizing the strain. The method specifically comprises steps as follows: a bacterium liquid with OD600 higher than 2.0 ppm is taken and inoculated to to-be-treated ammonia-nitrogen wastewater with the ammonia-nitrogen concentration not higher than 2,000 ppm, fermentation is performed while dissolved oxygen is maintained to be 2.0 ppm or higher, and the ammonia-nitrogen wastewater can be treated. The ammonia-nitrogen and total nitrogen removal effect is remarkable, and nitrite nitrogen and nitrate nitrogen are not increased. The method is simple and has good application value in a sewage treatment engineering.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a bacterial strain with heterotrophic nitrification and aerobic denitrification functions, and also relates to the application of the bacterial strain. Background technique [0002] In this century, environmental problems are a major problem threatening human existence, especially in developing countries. Recent research reports have shown that the nitrogen content emitted into the environment by human industrial production, agricultural chemical fertilizer application, and fossil fuel combustion is significantly increasing, and these excessive nitrogen emissions will cause great environmental problems, such as N 2 O will lead to global warming, and the increase of nitrogen levels in water bodies will lead to eutrophication of water bodies, threatening human health. Ammonia nitrogen, as an important inorganic substance in the process of nitrogen cycle, is one of the chi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N1/20
Inventor 宋立岩雷禹王洋清唐薇
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI