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Specimen Holders for Atomic Force Microscopy

A technology of sample, flexible support, applied in the field of sample holder, which can solve the problem of no large intact tissue attachment, hydration and positioning improvement

Active Publication Date: 2022-02-08
UNIVERSITY OF BASEL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, on the other hand, little improvement in the attachment, hydration and positioning of large intact tissues under AFM
Current protocols are limited to a variety of different bonding processes on hydrophobic (PTFE) supports or tissue culture dishes

Method used

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  • Specimen Holders for Atomic Force Microscopy
  • Specimen Holders for Atomic Force Microscopy
  • Specimen Holders for Atomic Force Microscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0069] according to Figures 1 to 4 , the sample holder 1 according to the invention comprises a flexible support 10, in particular made of PTFE, in the form of a strip comprising an upper side 10a and a lower side 10b. The sample holder 1 further comprises a plurality of elongated first holding assemblies 100 and second holding assemblies 200, wherein the holding assemblies 100, 200 each comprise a first portion 101, 201 formed of a metal strip 101, 201 and formed by a pointed end. The second part 102 , 202 is formed by the spikes 105 , 205 of 103 , 203 , wherein the spikes 105 , 205 are attached, in particular glued, to the respective metal strip 101 , 201 . The retaining assemblies 100 , 200 each extend along a longitudinal axis L, ie the first portion 101 , 102 and the second portion 201 , 202 of the retaining assemblies 100 , 200 extend along the longitudinal axis L. As shown in FIG. The retaining assemblies 100 , 200 are further connected, in particular glued, to the up...

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PUM

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Abstract

The invention relates to a sample holder (1) for holding a sample, in particular for use with an atomic force microscope. According to the invention, the sample holder (1) preferably comprises: a flexible support (10) having an upper side (10a) and a lower side (10b) facing away from said upper side (10a); a first and a second holding assembly (100, 200), wherein each holding assembly (100, 200) includes a first portion (101, 201) and an opposite second portion (102, 202), the first portion (101, 201 ) is preferably connected to the upper side (10a) of the flexible support (10), the second portion (102, 202) forming the tip (103, 203) of the respective holding assembly (100, 200), such that the holding assembly (100, 200) are each movable from a first position, in which the tips (103, 203) are placed adjacent to each other, into a second position, in which the tips (103, 203) are closer to each other than in the second position. further spaced apart from said first position and separated by a gap (G) for receiving at least a portion of the sample (S) to be held, and wherein said tips (103, 203) are designed to penetrate or squeeze The sample (S) is pressed to hold the sample (S) when the sample (S) is received by said gap (G) and the holding assembly (100, 200) is moved from the second position back into the first position.

Description

technical field [0001] The present invention relates to a sample holder for holding a sample, in particular a sample holder for use with an atomic force microscope, a corresponding system, and a method for holding such a sample. Background technique [0002] Atomic force microscopy (AFM) is today considered very promising and an important tool for in vivo and / or in vitro exploration of various biological processes and pathologies [1-5]. However, working with living biological tissues requires specific experimental setups to ensure their viability and subsequent precise and reproducible measurements. These settings include fast and easy sample transfer from the extraction location to the AFM, strong and easy sample attachment, and nearby physiological experimental conditions (gas, temperature, and perfusion). So far, much of the work has been done to elevate the experimental conditions as much as possible to a physiological environment. Currently, samples are typically tran...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01Q30/20B82Y35/00
CPCG01Q30/20
Inventor 马尔科·洛帕里奇
Owner UNIVERSITY OF BASEL