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Application of antiviral double-stranded RNA and its temperature-sensitive gel preparation in the treatment and prevention of diseases caused by hsv-1

A technology of HSV-1 and gel preparation, which is applied in the field of application of antiviral double-stranded RNA and its temperature-sensitive gel preparation in the treatment and prevention of diseases caused by HSV-1, and can solve damage to the nervous system and digestive system and other problems, to achieve the effect of not easy to degrade, increase transfection efficiency, and enhance drug efficacy

Active Publication Date: 2020-02-14
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, taking these nucleoside drugs can produce various side effects, such as damage to the nervous system and digestive system

Method used

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  • Application of antiviral double-stranded RNA and its temperature-sensitive gel preparation in the treatment and prevention of diseases caused by hsv-1
  • Application of antiviral double-stranded RNA and its temperature-sensitive gel preparation in the treatment and prevention of diseases caused by hsv-1
  • Application of antiviral double-stranded RNA and its temperature-sensitive gel preparation in the treatment and prevention of diseases caused by hsv-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1, the preparation of small nucleic acid of the present invention

[0064] The small nucleic acid of the present invention is artificially synthesized, and the sequence is as follows:

[0065] siRNA: its sense strand sequence is sequence 1 (5'GCCCAGAGGUUCCUCUUUA 3') in the sequence listing, and its antisense strand sequence is sequence 2 (5'UAAAGAGGAACCUCUGGGC3') in the sequence listing; the double-stranded nucleic acid uses U and C bases 2' methoxy modification.

Embodiment 2

[0066] Embodiment 2, preparation of temperature-sensitive gel and its effect detection

[0067] 1. Preparation of thermosensitive gel

[0068] Components and concentrations of thermosensitive gels:

[0069]

[0070] 2. Prepare small nucleic acid temperature-sensitive gels according to the above-mentioned component concentrations, and the specific methods are as follows:

[0071] 1) Weigh 170.0g of Poloxamer 407 and 2.0g of hydroxypropyl methylcellulose (HPMC E-50lv, Shanghai Colorcon Company, Lot No: TK16012401), add 500ml of distilled water (about 4°C), and stir for 5min , placed at 4°C to fully swell, disperse evenly, and dissolve to obtain a clear solution of a hydrophilic gel.

[0072] 2) Take 5.0g of collagen (purchased from Hubei Kangbaotai Fine Chemical Co., Ltd.) and add 50ml of distilled water, stir at room temperature for 5min, and place it for use.

[0073] 3) Take 10.0g of HP-β-CD and add 100ml of distilled water to dissolve, heat to 50°C to swell and wait fo...

Embodiment 3

[0077] Example 3, Preparation of temperature-sensitive gel and its effect detection

[0078] 1. Preparation of temperature-sensitive gel

[0079] Components of temperature-sensitive gels and their concentrations:

[0080]

[0081] 2. Prepare the above-mentioned component concentration temperature-sensitive gel, the specific method is as follows:

[0082] 1) Weigh 170.0g of poloxamer 407 and 5.0g of methylcellulose, add 500ml of distilled water (at about 4°C), stir for 5 minutes, and place it at 4°C to fully swell, disperse evenly, and dissolve to obtain hydrophilic Clear solution of gel.

[0083] 2) Add 5.0g of collagen to 50ml of distilled water, stir at room temperature for 5min, and place it for use.

[0084] 3) Take 20.0g of HP-β-CD and add 200ml of distilled water to dissolve, heat to 50°C to swell and wait for use.

[0085] 4) Add 207.5g of 1,2-propanediol into the gel solution prepared in step 1) and stir and mix evenly. Under stirring, slowly add the solutions o...

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Abstract

The invention discloses an antiviral double-stranded RNA and application of temperature-sensitive type gel prepared by the same in treating and preventing HSV-1 caused diseases. One strand of the double-stranded RNA is of a sequence 1 in the sequence table and the other strand thereof is of a sequence 2. Modified compound of the double-stranded RNA refers to substance obtained by chemically modifying the double-stranded RNA with 2' fluoro, 2' methoxyl, sulpho or 2' deoxidization or modifying the double-stranded RNA with cholesterol. According to tests, the temperature-sensitive type gel which is applied to skins and comprises active ingredient namely the double-stranded RNA can suppress foreign HSV-1expression of epithelial cells of the skins; thus, the temperature-sensitive type gel is applied to suppression of HSV-1 virus infection and treatment of HSV-1 caused diseases.

Description

technical field [0001] The invention relates to the application of antiviral double-stranded RNA and its temperature-sensitive gel preparation in the treatment and prevention of diseases caused by HSV-1 in the field of biotechnology. Background technique [0002] Herpes simplex virus type I (HSV-1) infection often causes herpes and sores on the mucous membranes of the mouth and genitals. Human is the only host of HSV-1, and about 80-90% of the population has been infected with HSV-1. HSV-1 mainly causes stomatitis and herpes of the lips, but more and more studies have found that HSV-1 can also cause genital diseases, and is a more common cause of genital diseases. It has been generally believed that more than 70% of genital herpes is caused by herpes simplex virus type 2 (HSV-2). According to the epidemiological survey in the past 10 years, the genital herpes caused by HSV-1 infection is increasing year by year. The latest data from the Americas, Europe and Asia show that...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K31/713A61K9/06A61P31/22
CPCA61K9/06A61K31/713A61K47/38A61K47/40A61K47/42
Inventor 张雅鸥王子强繁萍苏宏瑞卢锦华仇伟王凡张佩琢
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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