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A cell immunomagnetic bead sorting system

A technology of magnetic bead sorting and cell immunity, applied in the field of cell sorting, can solve the problems of inability to effectively guarantee the speed and efficiency of cell sorting, and inability to guarantee any capture efficiency.

Active Publication Date: 2018-04-24
SHANGHAI BAIZE MEDICAL APP & INSTR CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The magnetic field is only used to capture the sample bound to the magnetic beads, and cannot make any guarantee for the capture efficiency
Due to the limitations of the design of the magnetic field itself, the above device cannot effectively guarantee the speed and efficiency of cell sorting

Method used

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  • A cell immunomagnetic bead sorting system
  • A cell immunomagnetic bead sorting system
  • A cell immunomagnetic bead sorting system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0156] This embodiment describes the implementation of CD3 cell sorting experiments:

[0157] (1) Rinse the pipeline first. In the instrument, only the pinch valves 1, 7, and 10 are opened, the magnet is in a separated state, and the peristaltic pump rotates counterclockwise (rotating speed 4ml / min) to ensure that the buffer solution fills the entire pipeline, which helps to reduce the damage to the cells during the sorting process;

[0158] (2) Perform sample mixing and incubation. Inject a certain amount of buffer solution into the positive sample collection chamber as a solvent for the removal of the magnetic beads later. Only the pinch valves 3 and 6 are opened, the peristaltic pump rotates counterclockwise (rotating speed 2ml / min), and the sample is added to the mixing chamber; only the pinch valves 2 and 6 are opened, the peristaltic pump rotates counterclockwise (rotating speed 2ml / min), Slowly add magnetic beads into the mixing chamber; only the pinch valves 1 and 6 ...

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Abstract

The invention relates to a device used for cellular immunomagnetic bead sorting. The device comprises a driving unit, a magnetic sorting unit and control switches from first to ninth described in the invention; the driving unit is used for driving fluid to flow in a pipeline and provided with a first connector and a second connector, and the first connector and the second connector are used for being communicated with a first connector and a second connector of a mixing container for mixing a sample and magnetic beads respectively; the magnetic sorting unit is used for generating magnetic force to sort cells combined with the magnetic beads.

Description

technical field [0001] The invention belongs to the field of cell sorting, and in particular relates to a cell immune magnetic bead sorting system. Background technique [0002] Magnetic bead enrichment or separation is an emerging molecular and cell biology technology at the end of the last century, see US Patents US5411863 and US5385707 for details. This technology has been widely used in molecular biology nucleic acid extraction, solid-phase cDNA library construction, protein and cell sorting or enrichment. First, specific ligands or antibodies are bound to the surface of magnetic beads by means of biochemical technology. The working principle is to mix the magnetic bead suspension of the above-mentioned surface-bound ligand (antibody) with the target molecule or cell solution (suspension) to be selected, and through the ligand-receptor binding reaction or antigen-antibody reaction, the magnetic beads and the target molecule Or cells are tightly bound; by magnetically c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53C12M1/42C12M1/38C12M1/36
CPCC12M23/44C12M41/00C12M41/12C12M47/04G01N33/53
Inventor 钱其军周进师传胤庞震国胡金伟吕功路颜开冬
Owner SHANGHAI BAIZE MEDICAL APP & INSTR CO LTD
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