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Application of SNP molecular marker of rice grain cadmium content-related gene LCd-31

A rice grain and rice technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of yield impact, unfit for food, long and difficult treatment of cadmium-contaminated farmland, etc.

Active Publication Date: 2016-06-01
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In cadmium-contaminated cultivated land, 5-10% of the area yield is seriously affected, and the food produced is not suitable for consumption
The safety of food crops is closely related to human health, but the treatment cycle of cadmium-contaminated farmland is long and difficult. Therefore, how to produce safe food crops without wasting existing farmland is particularly important

Method used

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  • Application of SNP molecular marker of rice grain cadmium content-related gene LCd-31
  • Application of SNP molecular marker of rice grain cadmium content-related gene LCd-31
  • Application of SNP molecular marker of rice grain cadmium content-related gene LCd-31

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Embodiment 1

[0034] Example 1, TagSNP-9949648 is a SNP molecular marker associated with rice grain cadmium content

[0035] 1. Discovery of TagSNP-9949648

[0036]According to the cadmium content in the grains of 65 domestic and foreign rice varieties and the results of genome sequencing, the inventors of the present invention screened out a SNP site related to the cadmium content in rice grains. This SNP site corresponds to chromosome 9 of Nipponbare Rice. 9949648, the SNP site was named TagSNP-9949648. TagSNP-9949648 is a biallelic polymorphic SNP site of the 32nd nucleotide of SEQ ID No. 1 on rice chromosome 9, and the 32nd nucleotide of SEQ ID No. 1 is C or T. The 32nd position of SEQIDNo.1 (TagSNP-9949648) is the homozygous TagSNP-9949648 genotype named TT, and the 32nd position of SEQIDNo.1 (TagSNP-9949648) is the homozygous TagSNP-9949648 of C The genotype was designated as CC, and the TagSNP-9949648 genotype in which the 32nd position of SEQ ID No. 1 (TagSNP-9949648) is heterozyg...

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Abstract

The invention discloses application of an SNP molecular marker of a rice grain cadmium content-related gene LCd-31. The application specifically can be application of TagSNP-9949648 to the rice grain cadmium content identification or auxiliary identification; the TagSNP-9949648 is an SNP site of a rice genome and is 32<nd> nucleotide positioned on a 9 chromosome of rice and shown as SEQ ID No. 1, and the 32<nd> nucleotide is C or T. Experiments prove that the content of grain cadmium in 93 percent of rice variety with a genotype TT is lower than 0.2 mg / kg, the content of grain cadmium in 89 percent of rice variety with a genotype CC is higher than 0.2 mg / kg; the TagSNP-9949648 is indicated to be the rice grain cadmium content-related SNP molecular marker.

Description

technical field [0001] The invention relates to the application of SNP molecular markers of rice grain cadmium content-related genes in the field of biotechnology, in particular to the application of SNP molecular markers of rice grain cadmium content-related genes LCd-31. Background technique [0002] Single nucleotide polymorphism (singlenucleotide polymorphism, SNP) refers to the variation of a single nucleotide in the genome. It is the smallest variation unit and is a variation form formed by a single nucleotide pair substitution, transversion, insertion or deletion. [0003] Rice is one of the most important food crops in the world, and more than half of the population uses rice as a staple food. Heavy metal pollution in farmland is a major environmental problem facing the world today and has received widespread attention from all walks of life. Heavy metal pollution is complex, concealed, and persistent, and may endanger human health through the enrichment of the food...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 何振艳麻密许文秀闫慧莉
Owner INST OF BOTANY CHINESE ACAD OF SCI
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