Preparation and application of alpha fetoprotein electrochemical immunosensor based on silver deposition

An immune sensor and alpha-fetoprotein technology, applied in the direction of material electrochemical variables, material inspection products, biological testing, etc., can solve the problems of high price, complicated operation, unsuitable for real-time detection, etc., achieve high sensitivity, increase sensitivity, and promote The effect of electron transfer

Inactive Publication Date: 2016-06-01
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These analysis methods usually have the disadvantages of complicated operation, high price and not suitable for real-time detection

Method used

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  • Preparation and application of alpha fetoprotein electrochemical immunosensor based on silver deposition
  • Preparation and application of alpha fetoprotein electrochemical immunosensor based on silver deposition

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Experimental program
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Embodiment 1

[0032] Embodiment 1 A method for preparing an AFP electrochemical immunosensor based on silver deposition:

[0033] (1) with Al 2 o 3Grind a glassy carbon electrode (GCE) with a diameter of 4 mm with polishing powder, ultrasonically clean it in ethanol and ultrapure water for 3 min, and dry it with nitrogen; use 10 mL of HAuCl with a concentration of 1.0 mmoL / L 4 The solution was used as the bottom solution, GCE was used as the working electrode, the Ag / AgCl electrode was used as the reference electrode, and the platinum electrode was used as the counter electrode. After 30 circles of cyclic voltammetry scanning in the potential range of -0.8 ~ 0.6V, the electrode was placed at 0.1moL / L Soak in KCl solution overnight, wash with PBS (pH7.4) buffer solution to obtain gold nanocluster modified glassy carbon electrode (nano-Au / GCE);

[0034] (2) Take 10 μL of 1.0 μg / mL AFP primary antibody (Ab 1 ) standard solution onto the surface of nano-Au / GCE, incubated in a refrigerator at...

Embodiment 2

[0038] Embodiment 2 A method for preparing an AFP electrochemical immunosensor based on silver deposition:

[0039] (1) with Al 2 o 3 Grind a glassy carbon electrode (GCE) with a diameter of 4 mm with polishing powder, ultrasonically clean it in ethanol and ultrapure water for 3 min, and dry it with nitrogen; use 10 mL of HAuCl with a concentration of 1.0 mmoL / L 4 The solution was used as the bottom solution, GCE was used as the working electrode, the Ag / AgCl electrode was used as the reference electrode, and the platinum electrode was used as the counter electrode. After 30 circles of cyclic voltammetry scanning in the potential range of -0.8 ~ 0.6V, the electrode was placed at 0.1moL / L Soak in KCl solution overnight, wash with PBS (pH7.4) buffer solution to obtain gold nanocluster modified glassy carbon electrode (nano-Au / GCE);

[0040] (2) Take 10 μL of 2.0 μg / mL AFP primary antibody (Ab 1 ) standard solution onto the surface of nano-Au / GCE, incubated in a refrigerator a...

Embodiment 3

[0044] Embodiment 3 A method for preparing an AFP electrochemical immunosensor based on silver deposition:

[0045] (1) with Al 2 o 3 Grind a glassy carbon electrode (GCE) with a diameter of 4 mm with polishing powder, ultrasonically clean it in ethanol and ultrapure water for 3 min, and dry it with nitrogen; use 10 mL of HAuCl with a concentration of 1.0 mmoL / L 4 The solution was used as the bottom solution, GCE was used as the working electrode, the Ag / AgCl electrode was used as the reference electrode, and the platinum electrode was used as the counter electrode. After 30 circles of cyclic voltammetry scanning in the potential range of -0.8 ~ 0.6V, the electrode was placed at 0.1moL / L Soak in KCl solution overnight, wash with PBS (pH7.4) buffer solution to obtain gold nanocluster modified glassy carbon electrode (nano-Au / GCE);

[0046] (2) Take 10 μL of 2.5 μg / mL AFP primary antibody (Ab 1 ) standard solution onto the surface of nano-Au / GCE, incubated in a refrigerator a...

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Abstract

The invention relates to the technical field of electrochemical immunosensors, in particular to preparation and application of an alpha fetoprotein electrochemical immunosensor based on silver deposition. To be specific, gold nanoclusters are used to modify the surface of a glassy carbon electrode to obtain an antibody capture base, C60 supported gold nano composite traces and labels an alpha fetoprotein secondary antibody, via sandwiched immunoreaction, secondary antibody functional gold nanoparticles are captured to the surface of the sensor and induce silver deposition, and alpha fetoprotein is detected by detecting an electrochemical dissolving signal of silver nanoparticles in a KCl solution.

Description

technical field [0001] The invention relates to the technical field of electrochemical immunosensing, in particular to the preparation and application of an alpha-fetoprotein (AFP) electrochemical immunosensor based on silver deposition. Specifically, gold nanoclusters were used to modify the surface of glassy carbon electrodes as antibody capture substrates, C 60 The gold nanocomposite was loaded with the secondary antibody labeled AFP, and through the sandwich immunoreaction, the gold nanometer functionalized by the second antibody was captured on the surface of the sensor and induced silver deposition, which was realized by detecting the electrochemical dissolution signal of the silver nanometer in KCl solution Detection of AFP. Background technique [0002] Sensitive and accurate detection of tumor markers plays a vital role in the early treatment and diagnosis of diseases. The content of tumor markers in serum, tissue, and saliva can reflect the physiological state of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N27/48
CPCG01N33/68G01N27/48
Inventor 郑香丽周长利夏方诠田栋花小霞乔雪莹
Owner UNIV OF JINAN
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