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A complete medium for the induction of somatic embryos from pagoda tree cotyledons

A technology for inducing culture medium and somatic embryos, applied in the field of complete culture medium, can solve the problem of low regeneration rate of Sophora japonica, and achieve the effect of saving time

Active Publication Date: 2018-05-25
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the above problems, and to provide a complete set of culture medium for the induction of somatic embryos from the cotyledons of Sophora japonica, to solve the problem of low regeneration rate of Sophora japonica

Method used

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  • A complete medium for the induction of somatic embryos from pagoda tree cotyledons
  • A complete medium for the induction of somatic embryos from pagoda tree cotyledons
  • A complete medium for the induction of somatic embryos from pagoda tree cotyledons

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Select the mature seeds of Sophora japonica that are healthy and free from diseases and insect pests, remove the fruit pods, wash carefully with detergent, rinse with running water for 1 hour, filter paper to absorb excess water outside the seeds, and prepare for disinfection.

[0035] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 8 minutes, rinse with sterile water for 5 times, and dry the water with sterile filter paper , Gently peel off the seed coat with a scalpel blade and tweezers, and take out the cotyledons. Gently separate the two cotyledons with tweezers and a blade, keep the germ, then place the cotyledon with the flat side down and the convex side up in a petri dish, and use a scalpel to make 3-4 cuts perpendicular to the main vein of the convex side of the cotyledon. Take care not to scratch the edge of the cotyledon.

[003...

Embodiment 2

[0046] Select the mature seeds of Sophora japonica that are healthy and free from diseases and insect pests, remove the fruit pods, wash carefully with detergent, rinse with running water for 1 hour, filter paper to absorb excess water outside the seeds, and prepare for disinfection.

[0047] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 10 minutes, rinse with sterile water for 5 times, and dry the water with sterile filter paper , Gently peel off the seed coat with a scalpel blade and tweezers, and take out the cotyledons. Gently separate the two cotyledons with tweezers and a blade, keep the germ, then place the cotyledon with the flat side down and the convex side up in a petri dish, and use a scalpel to make 3-4 cuts perpendicular to the main vein of the convex side of the cotyledon. Take care not to scratch the edge of the cotyledon.

[00...

Embodiment 3

[0057] Select the mature seeds of Sophora japonica that are healthy and free from diseases and insect pests, remove the fruit pods, wash carefully with detergent, rinse with running water for 1 hour, filter paper to absorb excess water outside the seeds, and prepare for disinfection.

[0058] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 12 minutes, rinse with sterile water for 5 times, and dry the water with sterile filter paper , Gently peel off the seed coat with a scalpel blade and tweezers, and take out the cotyledons. Gently separate the two cotyledons with tweezers and a blade, keep the germ, then place the cotyledon with the flat side down and the convex side up in a petri dish, and use a scalpel to make 3-4 cuts perpendicular to the main vein of the convex side of the cotyledon. Take care not to scratch the edge of the cotyledon.

[00...

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Abstract

The invention discloses a complete set culture medium for the induction of cotyledon somatic embryos of Chinese scholartree. The complete set culture medium comprises a somatic embryo induction culture medium, an adventitious bud induction culture medium, a strong seedling culture medium and a rooting culture medium. When the culture medium disclosed by the invention is used for propagating Chinese scholartree seedlings, one step of establishing a Chinese scholartree rapid propagation and regeneration system can be saved, so that time is greatly shortened. The regeneration rate is high, more buds are germinated, and the plant transplanting survival rate can reach more than or equal to 90%; germchits grow healthily; and the germplasm degeneration problem of the high-quality germchits can be effectively solved, and an ideal receptor system also can be provided for genetic transformation or mutation breeding of the Chinese scholartree.

Description

technical field [0001] The invention relates to a complete culture medium for inducing somatic embryos of pagoda tree cotyledons, belonging to the field of plant biotechnology. Background technique [0002] Sophora japonica Linn, also known as Sophora japonica Linn and Chinese Sophora japonica Linn, belongs to Papilionaceae and Sophora genus. It is an ideal street tree species and garden greening tree species. Moreover, the material of Sophora japonica is excellent, the flowers, fruit, root bark, and bark can be used as medicine, and the seeds can be squeezed to make soap. It has high economic value and has a very broad application prospect. At the same time, the national pagoda tree is the only rootstock tree species for grafting ornamental species such as Sophora japonica, Jinzhi Huai, Liaohong Huai, and Butterfly Huai, and there is a large demand for seedlings. Sophora japonica is mainly propagated by seeds, but its flowering and fruiting have the phenomenon of large and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04C12N5/00A01H4/00
CPCA01H4/00A01H4/001
Inventor 夏阳李双云杨国良刘盛芳庞彩红孙超王守国刘瑞梅
Owner SHANDONG FOREST SCI RES INST
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