A method for uva to regulate the content of naringin in Quercus fern

A Quercus pomelo and content technology, which is applied in the field of plant cultivation, can solve problems such as restricting production practices, and achieve the effects of promoting production practices, alleviating ecological pressure, and speeding up the implementation process.

Active Publication Date: 2018-06-29
INST OF BOTANY CHINESE ACAD OF SCI
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  • Description
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AI Technical Summary

Problems solved by technology

Through the quality analysis of the artificially cultivated 3-year-old live oak fern, our research group found that the content of new eriocitrin was less than 0.1%, which severely limited the production practice of artificially cultivated oak fern as drynaria.
No relevant researchers or manufacturers at home and abroad have regulated the content of naringin in Quercus fern

Method used

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  • A method for uva to regulate the content of naringin in Quercus fern
  • A method for uva to regulate the content of naringin in Quercus fern
  • A method for uva to regulate the content of naringin in Quercus fern

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Experimental program
Comparison scheme
Effect test

Embodiment 180

[0031] Example 1 80KJ / m 2 Effects of UVA irradiated Quercus fern on the content of naringin and eriodinin

[0032] Take 6 3-year-old oak fern plants with the same growth potential, 3 of them are used as controls, and the other 3 plants are irradiated under LED light source UVA. When the radiation dose reaches 80KJ / m 2 stop processing. The contents of the two main active components, naringin and eriodinin, were analyzed by HPLC, and the injection volume was 40 μL. Get the chromatogram and analyze the contents of naringin and eriodinin in Quercus fern.

[0033] Table 1

[0034]

[0035] According to SPSS analysis, LED light source UVA 80KJ / m 2 After treatment with a certain radiation dose, there was no significant difference in the total content of naringin, eriocitrin and the two components in Quercus fern compared with the control. According to SPSS analysis, LED light source UVA 80KJ / m 2 After the radiation dose treatment, the total content of naringin and the two co...

Embodiment 2

[0036] Example 2 160KJ / m 2 Effects of UVA irradiated Quercus fern on the content of naringin and eriodinin

[0037] Take 6 3-year-old oak fern plants with the same growth potential, 3 of them are used as controls, and the other 3 plants are irradiated under LED light source UVA. When the radiation dose reaches 160KJ / m 2 stop processing. The contents of the two main active components, naringin and eriodinin, were analyzed by HPLC, and the injection volume was 40 μL. Obtain chromatograms and analyze the contents of naringin and eriodinin in Quercus fern.

[0038] Table 2

[0039]

[0040] According to SPSS analysis, LED light source UVA 160KJ / m 2 After treatment with a certain radiation dose, the content of naringin in Quercus fern decreased, and eriontin increased significantly, which was 5.4 times that of the control, and the total content of the two components had no significant difference compared with the control. It can be seen that the LED light source UVA 160KJ / m...

Embodiment 3

[0041] Example 3 320KJ / m 2 Effects of UVA irradiated Quercus fern on the content of naringin and eriodinin

[0042] Take 6 3-year-old oak fern plants with the same growth potential, 3 of them are used as controls, and the other 3 plants are irradiated under LED light source UVA. When the radiation dose reaches 320KJ / m 2 stop processing. The contents of the two main active components, naringin and eriodinin, were analyzed by HPLC, and the injection volume was 40 μL. Get the chromatogram and analyze the contents of naringin and eriodinin in Quercus fern.

[0043] table 3

[0044]

[0045]

[0046]According to SPSS analysis, after the radiation dose of LED light source UVA 320KJ / m2, the content of naringin in Quercus fern decreased, and the content of eriocitrin increased significantly, which was 5.0 times that of the control, and the total content of the two components was compared with that of the control There was no significant difference. It can be seen that the L...

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Abstract

The invention discloses a method for regulating and controlling the naringin content in drynaria roosii with UVA. Drynaria roosii plants are radiated at the radiation dose of 80-320 KJ / m<2> so that naringin in drynaria roosii can be regulated and controlled to be converted into neoeriocitrin. Compared with a control group not treated with the method, the naringin content in a treatment group is decreased by 56%, the neoeriocitrin content is increased by 5.4 times, and the total quantity of the two ingredients has no significant difference from that of the control group. By means of the method, naringin in drynaria roosii is effectively regulated and controlled to be converted into neoeriocitrin with higher medicinal value. The method is economical, practical, simple and convenient to implement and can be applied to regulating and controlling active ingredients in artificially-cultivated drynaria roosii, so that the production practice of using artificially-cultivated drynaria roosii as the rhizoma drynariae medicinal material is promoted, ecological pressure is relieved, large-scale development, utilization and popularization of the traditional Chinese medicine rhizoma drynariae are promoted, and the implementation progress of modernization of traditional Chinese medicine is accelerated.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for regulating the content of naringin in oak fern. Background technique [0002] Drynaria roosii is a medium-sized epiphytic fern. Its rhizome is used as drynaria rhizome as medicine, and as the only authentic product of Drynaria roosii, it is recorded in the "Chinese Pharmacopoeia". . In 2001, the Chinese Academy of Chinese Medical Sciences successfully developed the traditional Chinese medicine Qianggu Capsule by extracting the active ingredient total flavonoids from Quercus fern on the basis of many years of clinical practice. At present, my country has a great demand for Drynaria officinalis medicinal materials, but the raw materials of Quercus fern are still all sourced from the excavation of wild resources, which not only wastes a lot of manpower and financial resources, but also causes irreversible damage to the ecological environment, so the artific...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/04
CPCA01G7/04Y02P60/14
Inventor 石雷黄凤杰李杨李东姚然王頔杨华
Owner INST OF BOTANY CHINESE ACAD OF SCI
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