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Method for improving induction efficiency of mitotic gynogenetic dihaploid of bastard halibut

A gynogenesis, double haploid technology, applied in biochemical equipment and methods, microbial determination/inspection, fish farming, etc. Effect

Active Publication Date: 2016-06-15
中国水产科学研究院北戴河中心实验站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This greatly restricts the application of double haploid technology in fish breeding

Method used

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  • Method for improving induction efficiency of mitotic gynogenetic dihaploid of bastard halibut
  • Method for improving induction efficiency of mitotic gynogenetic dihaploid of bastard halibut
  • Method for improving induction efficiency of mitotic gynogenetic dihaploid of bastard halibut

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Embodiment 1

[0036] A method for improving the double haploid induction efficiency of flounder mitotic gynogenesis, comprising the following steps:

[0037] A. Identification and screening of homozygosity; use 24 microsatellite markers with high recombination rate covering 24 linkage groups of flounder to identify the homozygosity of female broodstock, and screen the female parent with a homozygosity greater than 0.2000 for double haploid female Induction of nuclear development.

[0038] B. Collection of sperm and ovum: screen the female flounder with high homozygosity, put it lightly on the cushion, cover the whole body with a towel to prevent it from beating the cushion, and gently wipe off the dirt and dirt around its genital opening with a dry towel Water, gently and slowly squeeze forward from the back of the fish body along the gonads, squeeze the eggs into a 1000ml clean beaker, store them in the dark, and use them for later use; screen the male red sea bream in the peak breeding pe...

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Abstract

The invention provides a method for improving the induction efficiency of mitotic gynogenetic dihaploid of bastard halibut. Before the mitotic gynogenetic dihaploid is induced, the homozygosity of female parents is identified, parents with high homozygosity are screened, and then induction is performed, thereby improving the induction efficiency. By utilizing the method, the batch and scale induction of the mitotic gynogenetic dihaploid of the bastard halibut can be realized, so that a solid foundation is established for functional gene mining, quantitative character controlling site positioning and fine breed cloning of biological characters of the bastard halibut.

Description

technical field [0001] The invention belongs to the technical field of aquatic organisms, and in particular relates to a method for improving the efficiency of inducing double haploids in flounder mitotic gynogenesis. Background technique [0002] Mitotic gynogenesis is a special reproductive mode that uses radiation-inactivated sperm to activate the egg, and then applies physical or chemical methods to the egg to inhibit its cleavage during a specific period. Individuals obtained by inducing mitotic gynogenesis are called double haploids, which are completely homozygous for the genome. Double haploid is an extremely precious basic material for breeding. Based on double haploid, homozygous clonal lines and heterozygous clonal lines can be quickly established. Regardless of homozygous clonal lines or heterozygous clonal lines, they are the best materials for quantitative trait mapping, functional gene mining, and whole genome sequencing. Simulation experiments show that usi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00C12Q1/68
CPCA01K61/00C12Q1/6888Y02A40/81
Inventor 王桂兴侯吉伦张晓彦王玉芬刘海金于清海杨立更
Owner 中国水产科学研究院北戴河中心实验站