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A kind of hippocampal oxytocic agent and its coding gene

An amino acid, sequence technology, applied in the fields of biochemistry and molecular biology

Active Publication Date: 2019-05-31
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the artificial breeding industry of seahorses is in the early stage of development, and has not been able to achieve low-cost large-scale breeding to meet market demand

Method used

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  • A kind of hippocampal oxytocic agent and its coding gene
  • A kind of hippocampal oxytocic agent and its coding gene
  • A kind of hippocampal oxytocic agent and its coding gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Extraction of total RNA from the whole brain of the striated hippocampus

[0020] A sexually mature linear hippocampus (Hippocampus erectus), with a body length of 5-8 cm and a body weight of about 8-10 g, was anesthetized with 20 mg / L MS222 (ethyl m-aminobenzoate methanesulfonate) for about 2 minutes, then sampling was started, and the samples were separated. The whole brain tissue was taken out, quickly frozen in liquid nitrogen, and stored in a -80°C refrigerator for later use. The total RNA of the whole brain of the striated hippocampus was extracted by Trizol reagent method, and its OD 260 / 280 = 1.90.

[0021] 2. Cloning of kiss2 cDNA

[0022] 2.1. Synthesis of the first strand of cDNA

[0023] 10 μg of total RNA samples from the whole brain of the lined hippocampus were treated with DNAase I to remove the contamination of genomic DNA, mixed with RNA Oligo dT, and subjected to reverse transcription PCR. The first strand of cDNA obtained was stored at -20°C fo...

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Abstract

The invention discloses a sea horse oxytocic and an encoding gene thereof. An amino acid sequence of the sea horse oxytocic is shown in SEQ ID NO.2. An amino acid sequence of a hippocampus kelloggi kiss2 gene is shown in SEQ ID NO.1. According to the invention, a new sea horse oxytocic is separated from hippocampus kelloggi, the new sea horse oxytocic can activate pituitary to secrete follicle stimulating hormone and promote gonad to secrete sexual steroid hormone, so as to participate in reproductive regulation of sea horse, conditions are provided for solving sea horse larval rearing, and realization of large-scale sea horse culture can be benefited.

Description

Technical field: [0001] The invention belongs to the fields of biochemistry and molecular biology, and in particular relates to a hippocampus oxytocic agent and its coding gene. Background technique: [0002] The reproductive activities of vertebrates are mainly regulated by the reproductive axis of "brain / hypothalamus-pituitary-gonad" (brain-pituitary-gonad axis, BPG axis). The brain / hypothalamus secretes and produces gonadotropin releasing hormone (GnRH), which stimulates pituitary gonadotropin (gonadotropin, GtH), including luteinizing hormone (Luteinizing Hormone, LH) and follicle stimulating hormone (Follicle stimulating hormone) , FSH) synthesis and release, gonadotropins act on the gonads, increase the production and secretion of gonadal sex steroid hormones, and then promote the maturation of gonads, gamete production and discharge. Kisspeptin, the gene upstream of the reproductive axis (GnRH-GtH axis), is encoded by the kiss gene, and undergoes post-translational b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/46C12N15/12A61K38/17A61P5/06
CPCA61K38/00C07K14/461
Inventor 张辉贤林强张艳红秦耿罗伟
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI