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Follicle-stimulating hormone time-resolved fluoroimmunoassay method and kit

A follicle-stimulating hormone and immunofluorescence technology is applied in the detection field of time-resolved immunofluorescence analysis, which can solve the problems of poor stability, low sensitivity, polluted environment, etc., and achieve the effects of small cross-reaction, good specificity and high analytical sensitivity.

Inactive Publication Date: 2009-12-30
PERKINELMER MEDICAL DIAGNOSTICS PROD SHANGHAI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a time-resolved immunofluorescence analysis method and kit for FSH detection, which mainly solve the technical problems of low sensitivity, poor stability, cumbersome operation and environmental pollution in the prior art.

Method used

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  • Follicle-stimulating hormone time-resolved fluoroimmunoassay method and kit
  • Follicle-stimulating hormone time-resolved fluoroimmunoassay method and kit
  • Follicle-stimulating hormone time-resolved fluoroimmunoassay method and kit

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Embodiment

[0030] Follicle Stimulating Hormone (FSH) Determination

[0031] 1. Preparation of standard products

[0032] Use 6% BSA, 4% sugar, 0.1% NaN3, 50mmol / L Tris-HCl buffer solution to prepare FSH protein into 0, 3, 10, 30, 100, 300mIU / mL standard solution, and calibrate with national FSH standard. Aliquot and store at +2~+8℃.

[0033] 2. Operation steps

[0034] 1) Preparation of reagents

[0035] a) Washing liquid: Mix 40mL of concentrated washing liquid and 960mL of deionized water in a clean washing liquid bottle, and use it as a working washing liquid for later use.

[0036] b) Europium labeling: within one hour before use, dilute the europium label 1:20 with assay buffer.

[0037] c) Equilibrate the kit analysis buffer, the sample to be tested, the calibrator and the required number of microwell reaction strips to room temperature (20-25° C.).

[0038] 2) Pipette 25 μl of calibrator or sample, and add to the bottom of the corresponding microwell in sequence.

[0039] 3)...

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Abstract

The invention discloses a follicle-stimulating hormone (FSH) time-resolved fluoroimmunoassay method and a kit. The method comprises the following steps: selecting and using a monoclonal antibody against FSH as a coating antibody, and diluting sodium carbonate buffer solution to 1 to 10mu g / ml as coating liquid; matching FSH monoclonal antibody, labeling the FSH monoclonal antibody with a lanthanide iron, and taking the labeled FSH monoclonal antibody as a labeled antibody; diluting and using the reaction buffer solution according to a ratio of 1 to 20 during experiment; and on a reaction plate of the coating antibody, adding 25mu l of standard substance of FSH or sample to be detected and the diluted labeled antibody into each hole in turn, and carrying out fluorescence detection after hatching. The invention also provides the corresponding kit. The method and the kit have the advantages of high sensitivity, specificity and stability; and the high automated assay system can improve the speed of clinical examination result, greatly reduce human error and increase the reliability of detected result.

Description

Technical field: [0001] The invention relates to a kit for quantitative detection of follicle-stimulating hormone (FSH), which belongs to the detection field of time-resolved immunofluorescence analysis. Background technique: [0002] Follicle-stimulating-hormone (FSH) is a glycoprotein secreted by the anterior pituitary gland, composed of two polypeptide chains through non-covalent bonds, with a molecular weight of 37,000. FSH can enhance the synthesis of cytochrome P450, and convert androgen into estrogen, and FSH and LH jointly regulate the synthesis of steroid hormones in the ovary, and play a vital role in the maturation of follicles and the generation of corpus luteum. In women, FSH can promote follicle maturation, ovulation, and change synchronously with LH in the menstrual cycle, and in men, FSH mainly promotes the production and maturation of sperm. In menopause, after oophorectomy and premature ovarian failure, FSH levels increase, and at this time, giving estroge...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/577G01N21/64
Inventor 沈君吴冯波
Owner PERKINELMER MEDICAL DIAGNOSTICS PROD SHANGHAI
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