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A kind of preparation method of phosphorylcholine biomimetic coating

A phosphorylcholine and coating technology, which is applied in the field of material surface science and biomedical polymer materials, can solve the problem of difficult to obtain the surface of high-content phosphorylcholine groups, and the rigidity of fixed groups hinders phosphorylcholine groups Orientation migration, limited biocompatibility and other problems, to achieve the effect of improved hydrophilicity and blood compatibility, simple method and mild conditions

Inactive Publication Date: 2018-05-04
XIAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods obtain a relatively stable imitation cell outer membrane structure coating, the rigidity of the fixing group also hinders the maximum orientation migration of the phosphorylcholine group, making it difficult to obtain a high content of phosphorylcholine group clumped surface, which limits the further improvement of biocompatibility

Method used

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  • A kind of preparation method of phosphorylcholine biomimetic coating
  • A kind of preparation method of phosphorylcholine biomimetic coating
  • A kind of preparation method of phosphorylcholine biomimetic coating

Examples

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Embodiment 1

[0038] This embodiment includes the following steps:

[0039] Step 1. Under nitrogen protection conditions, dissolve 16mmol 2-methacryloyloxyethyl phosphorylcholine monomer and 4mmol 2-aminoethyl methacrylate hydrochloride monomer with distilled water and initiate at 0.1mmol The free radical polymerization reaction is carried out under the action of the agent, the reaction temperature of the free radical polymerization reaction is 70°C, and the reaction time is 12h. Freeze-drying under the condition of ℃ to obtain phosphorylcholine polymers containing amino groups; the initiator is potassium persulfate;

[0040] Step 2. Dissolve 1 mg of the amino group-containing phosphorylcholine polymer described in step 1 in 1 mL of methanol to obtain a polymer solution, then add 15 μL of glutaraldehyde aqueous solution to the polymer solution, mix well, and obtain a mixed Solution; The mass concentration of described glutaraldehyde aqueous solution is 50%;

[0041] Step 3. Apply the mixe...

Embodiment 2

[0044] Example 2 is the same as Example 1, except that the polycarbonate film obtained in step 3 with a phosphorylcholine biomimetic coating on its surface is heated for 12 hours under a vacuum condition of 110° C., and washed with distilled water Finally, the phosphorylcholine biomimetic coating after hydrophobic treatment was obtained.

Embodiment 3

[0056] This embodiment includes the following steps:

[0057] Step 1. Under nitrogen protection conditions, dissolve 14mmol of acryloyloxyethylphosphorylcholine monomer and 6mmol of 2-aminoethyl methacrylate hydrochloride monomer in distilled water under the action of 0.2mmol of initiator Carry out free radical polymerization, the reaction temperature of free radical polymerization is 60°C, and the reaction time is 20h. After the reaction, the reaction solution is concentrated, then dialyzed with a dialysis bag with a molecular weight cut-off of 6000D to 8000D, and then frozen at -50°C Dry to obtain phosphorylcholine polymers containing amino groups; the initiator is ammonium persulfate;

[0058] Step 2. Dissolve 0.5 mg of the amino group-containing phosphorylcholine polymer in 1 mL of methanol to obtain a polymer solution, then add 10 μL of glutaraldehyde aqueous solution to the polymer solution, and mix well to obtain Mixed solution; the mass concentration of the glutaralde...

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Abstract

The invention discloses a preparation method of a phosphorylcholine biomimetic coating, which comprises the following steps: 1. Under the action of an initiator, vinyl monomers containing phosphorylcholine groups and vinyl monomers containing amino groups are Carry out free radical polymerization to obtain phosphorylcholine polymers containing amino groups; 2. Dissolve the phosphorylcholine polymers containing amino groups in polar solvents to obtain polymer solutions, then add glutaraldehyde aqueous solution, and mix well , to obtain a mixed solution; 3. Apply the mixed solution on the surface of the material to be modified, and after drying, place the material to be modified in distilled water, and soak it for 6h to 12h at a temperature of 40°C to 80°C, After taking out, a phosphorylcholine biomimetic coating is obtained on the surface of the material to be modified. The method of the invention is simple and the conditions are mild, and it is expected to become a new way to regulate the interaction between biological materials and biologically active substances such as proteins and platelets, and has important academic significance.

Description

technical field [0001] The invention belongs to the technical fields of material surface science and biomedical polymer materials, and in particular relates to a preparation method of a phosphorylcholine bionic coating. Background technique [0002] When the material is used in a living body, it is easy to non-specifically adsorb proteins, activate complement molecules and the immune system, thereby causing blood coagulation, immune and inflammatory reactions, resulting in a significant decrease in its performance or even failure. This is due to the poor biocompatibility of materials, therefore, biocompatibility research has become the primary issue in the field of biomaterial research. The surface of a material is the medium through which the material contacts the organism. Surface charge, hydrophilicity / hydrophobicity, chemical composition, and morphology are important factors that affect the interface interaction between the material and the organism, and determine whethe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/04C08L69/00C09D143/02C09D7/63C08F230/02C08F220/34C08F220/60
CPCC08F230/02C08J7/0427C08J2369/00C08J2443/02C08K5/07C09D7/63C09D143/02C08F220/34C08F220/60
Inventor 张亚刚
Owner XIAN UNIV OF SCI & TECH