A kind of culture method of high-purity Nomura reyesi yeast cell

A technology of Nomura reinhardtii and a culturing method is applied in the field of high-purity Nomura reinhardtii yeast-type cell culture, and can solve the problem that yeast-type colony boundaries are not obvious, conidia dispersion effect is poor, yeast-type cell purity is not high, etc. problem, to achieve the effect of long duration, obvious boundary and high purity
CN105670952BInactive Publication Date: 2017-03-15齐肇然
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
齐肇然
Publication Date
2017-03-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a culture method for high-purity Nomuraea rileyi yeast type cells. The method comprises the following steps that a culture medium is prepared, Nomuraea rileyi conidiospores are evenly dispersed on the newly-prepared culture medium to obtain the culture medium inoculated with the Nomuraea rileyi conidiospores, the distribution density of the culture medium inoculated with the Nomuraea rileyi conidiospores is 0.5-3 / cm<2>, the inoculated culture medium is placed in the illumination environment under the temperature of 24-30 DEG C and the humidity of 75-90%, the semitransparent liquid drop shaped Nomuraea rileyi yeast type cells are obtained on the surface of the culture medium after 96-120 h, and then the Nomuraea rileyi yeast type cells are amplified and cultured. According to the culture method for the high-purity Nomuraea rileyi yeast type cells, the duration time of the growing period of the yeast type cells is long, and the conversion time of the yeast type cells to the hypha state is correspondingly prolonged, so that the yield of the yeast type cells is improved, no mycelium is detected in the microscope examination, and the purity is high.
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Description

technical field

[0001] The invention relates to the field of insecticidal fungal cells, in particular to a method for cultivating high-purity Nomura reyesi yeast cells. Background technique

[0002] As an entomogenous fungus, Nomura lyseri has become a hotspot in theoretical research and production application in the field of biological control. Nomura lyseri has two types of growth phenomena on the surface of solid medium, that is, the transition from yeast-type cell state to hyphae growth state. On the one hand, , the yeast-type cells of Nomura reyesi are similar to the worms in the worm body, and the blastospores produced in liquid fermentation grow rapidly and are suitable for large-scale industrial production; on the other hand, in the ATMT system developed by Shao Changwen, the yeast-type cells As a recipient cell, whether it is industrial production or laboratory research, it has put forward a realistic demand for the cultivation of Nomura reyesi yeast cells. [0003...

Claims

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