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Application of rice gene OsHPL3 in improving stress tolerance of plants

A technology of plant stress resistance and genetics, applied in the fields of application, plant products, genetic engineering, etc., to achieve broad application prospects and improve tolerance

Inactive Publication Date: 2016-06-15
FRONTIER LAB OF SYST CROP DESIGN CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on HPL in plant stress resistance, especially waterlogging resistance.

Method used

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  • Application of rice gene OsHPL3 in improving stress tolerance of plants
  • Application of rice gene OsHPL3 in improving stress tolerance of plants
  • Application of rice gene OsHPL3 in improving stress tolerance of plants

Examples

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Embodiment Construction

[0028] The method used in the following examples is a conventional method if there is no special instructions, and the specific steps can be found in: "Molecular Cloning: A Laboratory Manual" (Sambrook, J., Russell, DavidW., Molecular Cloning: A Laboratory Manual, 3 rd edition, 2001, NY, Cold Spring Harbor). The primers and DNA sequences used were synthesized by Shanghai Yingjun Biotechnology Co., Ltd.

[0029] 1. Isolation of OsHPL3 gene and construction of expression vector

[0030] Design primers according to the sequence of the gene in GenBank, design the 5' end primer from the coding start site ATG of the gene, and design the 3' end primer at the stop codon:

[0031] Forward primer: 5'GAATTCatggtgccgtcgttcccgc3' (SEQ ID NO: 5)

[0032] Reverse primer: 5'GGCGCGCCttagctgggagtgagctccc3' (SEQ ID NO: 6)

[0033] Using Zhonghua 11 cDNA as a template, the full-length gene of OsHLP3 was amplified by PCR with the above primers. The length of the gene is 1464bp, its nucleotide ...

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Abstract

The invention discloses an application of a rice gene OsHPL3 in improving the stress tolerance of plants. The experiment proves that after the gene disclosed by the invention is transferred into rice, the tolerance of the rice to adversity stress such as high salinity and drought can be obviously improved. The protein and an encoded gene of the protein provided by the invention have important theoretical and practical significances for the research on stress tolerance mechanisms of the plants, the improvement on the stress tolerance of the plants, and the improvement on the related characters of the plants, can play an important role in the stress tolerance genetic engineering improvement of the plants (especially the cereal crops), and has a wide application prospect.

Description

technical field [0001] The invention relates to a gene related to plant stress tolerance and its application, in particular to the application of the gene OsHPL3 derived from rice and related to stress resistance in improving plant stress tolerance. Background technique [0002] Rice, corn and wheat are important food crops in my country. The yield and quality of the three major crops are crucial to my country's food production and food security. Abiotic stresses such as drought, salinity and freezing will directly affect the normal growth and yield of food crops. Waterlogging stress is also one of the most serious natural disasters faced by human beings. my country is also a country with relatively serious flood disasters. About 2 / 3 of the country's land area has different degrees of waterlogging damage, which is extremely harmful. Understanding the mechanism of plant response to waterlogging stress and revealing its adaptation mechanism, so as to rationally select and cu...

Claims

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Application Information

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IPC IPC(8): C12N15/60C12N9/88C12N15/82A01H5/00
Inventor 唐晓艳周君莉陈竹锋刘东风王峥
Owner FRONTIER LAB OF SYST CROP DESIGN CO LTD