Chromatography purification and separation system and separation and purification method
A separation system and chromatographic purification technology, which is applied in the chromatographic purification separation system and the field of separation and purification, can solve problems such as cumbersome process, product degradation, increased impurities, and increased cost
Active Publication Date: 2016-06-22
河北海德生物科技有限公司
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Problems solved by technology
The process of this scheme is relatively cumbersome. For products with poor stability, multiple post-processing may lead to product degradation and increased impurities, and the cost will also increase.
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[0092] The chromatographic purification and separation system of the present invention is used to purify and separate the plant extract product, comprising the following steps:
[0093] 1) Firstly, fill 5 kilograms of 20 micron octadecylsilane bonded silica filler into the first chromatographic column 103 (column specification ID200x650mm) and fill 5 kilograms of 20 micron octadecane into the second chromatographic column 107 (column specification ID200x650mm) Based on silane-bonded silica gel packing, each use 20L of methanol: water = 50:50 (v / v) solvent to clean and balance the first chromatographic column and the second chromatographic column.
[0094] 2) Add 0.25 kg of the crude extract to 1 L of methanol:water=80:20 (v / v) solution, and the dissolved sample is filtered and injected into the first chromatographic column 103 .
[0095] 3) Then use the first chromatographic pump 102 to pump the configured methanol:water=50:50 (v / v) solvent (mobile phase) in the first solvent ...
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The invention provides a chromatography purification and separation system.The system comprises a first solvent inlet, a first chromatography pump, a first chromatographic column, a first detector, a second solvent inlet, a second chromatography pump, a second chromatographic column, a second detector, a first collecting port, a second collecting port, a third collecting port, a first three-way valve and a second three-way valve, wherein the first solvent inlet is communicated with the inlet of the first chromatographic column through the first chromatography pump; the outlet of the first chromatographic column is communicated with the first port of the first three-way valve through the first detector; the second port of the first three-way valve is communicated with the first collecting port, and the third port of the first three-way valve is communicated with the first port of the second three-way valve; the second port of the second three-way valve is communicated with the second collecting port, and the third port of the second three-way valve is communicated with the inlet of the second chromatographic column; the second solvent inlet is communicated with the inlet of the second chromatographic column through the second chromatography pump; the outlet of the second chromatographic column is communicated with the third collecting port through the second detector.The chromatography purification and separation system is simple and practicable and capable of increasing separation and purification effect and separation purity.
Description
technical field [0001] The invention relates to the field of industrial preparation chromatography, in particular to a chromatographic purification separation system and a separation and purification method. Background technique [0002] The main purpose of industrial preparative chromatography is to obtain pure substances from mixtures, and chromatographic techniques are often used for separation and collection to obtain one or more chromatographically pure substances. [0003] At present, the traditional chromatographic separation method includes the following steps: (1) cleaning the equilibrated chromatographic column; (2) injecting the dissolved and configured sample into the chromatographic column; (3) switching the mobile phase for elution, and collecting and preparing the spectrum; (4) ) Start receiving the target product after finding the target product on the preparation spectrum; (5) Detect the purity of the target product after the product is released. This tradi...
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IPC IPC(8): B01D15/10B01D15/42
CPCB01D15/10B01D15/426
Inventor 崔万臣
Owner 河北海德生物科技有限公司