Kit for detecting PIK3CA gene mutation

Abstract

The invention discloses a kit for detecting PIK3CA gene mutation, which comprises peptide nucleic acids and specific primers with the following sequences: (1) peptide nucleic acid and the specific primer aiming at the ninth exon of a PIK3CA gene: shown as SEQ ID NO.1-3; and (2) peptide nucleic acid and the specific primer aiming at the twentieth exon of a PIK3CA gene: shown as SEQ ID NO.4-6. A method for detecting the PIK3CA gene mutation by utilizing the kit comprises the following steps: (1) extracting the DNA of a to-be-detected sample; (2) by taking the extracted DNA as a template, carrying out quantitative PCR amplification by utilizing the kit for detecting the PIK3CA gene mutation, and detecting the value of deltaCt; and (3) making judgment, if deltaCt is greater than 8, judging that the genotype of the PIK3CA gene of the to-be-detected sample is the wild type, and if deltaCt is smaller than 8, judging that the genotype of the PIK3CA gene of the to-be-detected sample is the mutation type. The kit has the advantages that the operation is easy and convenient, the time consumed is short, the sensitivity is high, the detection result is accurate and reliable, etc.

Description

technical field [0001] The invention relates to a kit for detecting PIK3CA gene mutation. Background technique [0002] The PIK3CA gene was detected by Volinia in 1994 by in situ hybridization technique, and it encodes the p110 catalytic subunit of class I phosphatidylinositol-3-kinases (phosphatidylino-sitol3-kinases, PI3Ks). Mutations in exon 9 and exon 20 can not only reduce cell apoptosis but also promote tumor invasion and increase the activity of downstream kinases PI3Ks. Its mutation increases the activity of its downstream kinase PI3Ks, which can reduce cell apoptosis and promote tumor infiltration. At present, clinical detection methods need to amplify gene fragments first, and then use sequencing methods to detect gene mutations. The cost of this method is relatively high, and the detection cost is expensive. Moreover, since both the mutant gene and the wild gene are amplified to the same extent during the amplification of gene fragments, the sensitivity of sequ...

AI Technical Summary

Problems solved by technology

This method is costly and expensive to detect

Moreover, since both the mutant gene and the wild gene are amplified to the same extent during the amplification of gene fragments, the sensitivity of sequencing detection is not high, only about 10%, and the operation is cumbersome and time-consuming

In addition, for blood samples, it is difficult to detect mutations due to low sensitivity

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