A method for rapid screening of topoisomerase I inhibitors in natural products
A technology for topoisomerase and natural products, applied in the field of natural product analysis, can solve the problems of inability to provide detailed characteristics of protein-ligand interactions, false negatives, etc., and achieve fast sample analysis, strong specificity, and spectral information. large amount of effect
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Embodiment 2
[0143] The topoisomerase I inhibitor is a natural product extract or its monomer compound; the natural product Lycoris alkaloid extract of the present invention comprises the following steps:
[0144] ① Preparation of samples to be tested
[0145] Accurately weigh an appropriate amount of the sample to be tested, use the buffer solution prepared in Example 1 to fully dissolve, and the final concentration of the solution to be tested is 2.0 mg / ml;
[0146] Described sample to be tested is Lycoris alkaloid extract;
[0147] ② Preparation of standard solution
[0148] The internal standard sample was prepared into a standard stock solution with a concentration of 100 μg / ml with acetonitrile, and added to the sample solution to be tested before mass spectrometry detection, with a final concentration of 5 μg / ml;
[0149] Described internal standard sample is lotus leaf base;
[0150] ③ Binding reaction of the sample to be tested with normal and inactive topoisomerase I
[0151]...
Embodiment 3
[0174] "Chinese Pharmacopoeia", 2015 edition, stipulates that the total amount of diosgenin compounds diosgenin I and II and peninoside compounds diosgenin VI and VII in Chonglou medicinal materials shall not be less than 0.6% as a measure of its medicinal value. index. Zhongzhonglou in traditional Chinese medicine is often composed of prescriptions for the treatment of tumors, such as esophageal cancer, laryngeal cancer, rectal cancer, lung cancer, cervical cancer, leukemia, etc. However, there are few studies on the anti-tumor targets of Chonglou and its main medicinal components.
[0175] The topoisomerase I inhibitor is a natural product extract or its monomeric compound; the topoisomerase I inhibitor described in this embodiment is the monomeric compound trilobites I, II, VI and VII;
[0176] ① Preparation of samples to be tested
[0177]Accurately weigh an appropriate amount of the sample to be tested, first dissolve it with a small amount of dimethyl sulfoxide, and th...
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