A Data Modeling Method for DNA Amplification Process
A technology of data modeling and gene amplification, applied in the field of data modeling of gene amplification process, can solve problems such as the increase of ambient temperature, and achieve the effect of obvious processing effect.
Active Publication Date: 2018-03-20
南京宁创经纬智能技术有限公司
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[0002] The reciprocating process of DNA amplification is controlled by a computer. During the control process, due to the complex environmental factors in the reaction chamber, the ring temperature will increase with the increase in the number of cycles during the cycle process. Most control methods are sensitive to this change. The method of fan cooling is used to solve the problem, but the addition of the fan also has a certain degree of negative impact, such as the problem of cooling uniformity in the reaction chamber and the problem of interference noise. Therefore, these have attracted great attention from foreign research experts. The method improved the structure and sealing of the reaction chamber, and achieved certain results.
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[0025] The method of the present invention is based on three reaction stages of DNA amplification: denaturation, annealing and extension. In each reaction stage of DNA amplification, there are 6 reaction sections in total, each reaction section Ci, i=1, 2, 3 , 4, 5, 6 can be expressed as:
[0026] C i =[t i-1 , t i ]
[0027] The DNA amplification process is a process of cyclically executing the reaction stage, and the time period from the first reaction stage to the last reaction stage is called the reaction interval. represent the two endpoints of the reaction interval of the DNA amplification process respectively, then any time t in the reaction interval can be expressed as: Control reference points during the reaction phase of DNA amplification are called control nodes. There are four control nodes in each reaction stage, and the control nodes in the denaturation stage, annealing stage and extension stage can be expressed as g 1 , g 2 , g 3 , g 4 ,z 1 ,z 2 ,z ...
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The invention provides a data modeling method for the DNA amplification process.By means of the method, control parameters can be regulated and revised according to environment changes, all details of the DNA amplification process are analyzed and researched through a mathematical method, and the control flow can be classified according to the characteristics of DNA.In each reaction section, an independent control algorithm is formed according to a control mathematical model.Corresponding numerical experiments are conducted on control nodes such as 94, 72 and 45 of each section, and experiment results reflect validity of the control model.Six reaction sections are included in total, in the reaction process of 505 seconds, DNA reaction characteristics are truly recorded in each reaction section, and experiment curves truly reflect basic rules of gene amplification.The control mathematical model can accurately control each control node in the DNA amplification process, the intelligent DNA control process is achieved, and the model has wide adaptability.
Description
technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a data modeling method for a gene amplification process Background technique [0002] The reciprocating process of DNA amplification is controlled by a computer. During the control process, due to the complex environmental factors in the reaction chamber, the increase in the number of cycles will cause the ring temperature to rise during the cycle process. Most control methods are sensitive to this change. The method of fan cooling is used to solve the problem, but the addition of the fan also has a certain degree of negative impact, such as the problem of cooling uniformity in the reaction chamber and the problem of interference noise. Therefore, these have attracted great attention from foreign research experts. Methods The structure and sealing of the reaction chamber were improved, and certain effects were obtained. Contents of the invention [0003...
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IPC IPC(8): G06F19/12
CPCG16B5/00
Inventor 王金鹤吕剑庞丽萍
Owner 南京宁创经纬智能技术有限公司