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Application of D-chiro-inositol in preparation of hepatic fibrosis resisting medicine

A technology of anti-hepatic fibrosis and chiral inositol, applied in the field of medicine

Active Publication Date: 2016-07-13
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of D-chiroinositol in anti-hepatic fibrosis has not been reported so far at home and abroad.

Method used

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  • Application of D-chiro-inositol in preparation of hepatic fibrosis resisting medicine
  • Application of D-chiro-inositol in preparation of hepatic fibrosis resisting medicine
  • Application of D-chiro-inositol in preparation of hepatic fibrosis resisting medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] "Example 1" Preparation of Sprague-Dawley rat common bile duct ligation (BDL) liver fibrosis model and administration of D-chiroinositol

[0032] Sprague-Dawley male rats (body weight 180-200g) were randomly divided into sham operation group, BDL model group, D-chiroinositol (150mg / kg / day, dissolved in daily drinking water) administration group, and the sham operation group 5 rats, 9 rats in the BDL model group and 9 rats in the 16-4 administration group. Animals were fasted for 12 hours before the operation. After being anesthetized with isoflurane, the laparotomy was performed under aseptic conditions, the liver margin was raised, the duodenum was pulled apart, and the common bile duct was separated by 2-3 cm. Two 000-gauge silk sutures were ligated near the duodenum and near the porta hepatis, and the common bile duct was cut from the middle of the two ligatures, and the incision was sutured after restoring the liver to its original state. In the sham operation grou...

Embodiment 2

[0033] 《Example 2》Effect of D chiral inositol on serum liver function of BDL model rats.

[0034] Fasting for 12 hours before sampling, rats were anesthetized with 10% chloral hydrate, blood was collected from the abdominal aorta, left at room temperature for 1 hour, centrifuged at 3000rpm for 5 minutes, and serum was taken for detection of serum biochemical indicators. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are two major indicators of liver function. The results showed that the activities of AST (721.5±99.0vs222.4±25.4, pfigure 1 ), indicating that D-chiro-inositol can significantly improve the liver function of BDL rats.

Embodiment 3

[0035] "Example 3" Inhibitory Effect of D Chiroinositol on BDL-Induced Rat Liver Pathological Structural Changes

[0036] Before sampling, the rats were fasted for 12 hours, and the rats were sacrificed, and the liver tissue was taken, and the lobe of the liver was excised and fixed in neutral formaldehyde. After dehydration, paraffin embedding, slicing, baking slices, etc. to make paraffin sections. Hematoxylin-eosin (HE) staining was used to observe the changes of the pathological structure of rat liver tissue. The results showed that the hepatic cells in the liver tissue of rats in the sham operation group were arranged regularly, the hepatic lobule was intact, and there was no inflammatory cell infiltration and bile duct hyperplasia. significantly increased; D-chiro-inositol administration, rat liver tissue structure was significantly improved, bile duct hyperplasia was significantly inhibited, and the degree of tissue necrosis was significantly reduced ( figure 2 ). I...

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PUM

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Abstract

The invention belongs to the field of medicines, and relates to an application of D-chiro-inositol in preparation of a hepatic fibrosis resisting medicine. Systematic researches of the influence on blood serum liver function indexes AST and ALT activity of bile duct ligation (BDL) model group mice, the improvement on a liver pathological structure, the influence on the hepatic fibrosis degree and liver tissue hydroxyproline content, the influence on the blood serum and the liver tissue oxidative stress level, the influence on inflammatory reactions, and the like, prove that the D-chiro-inositol can inhibit the generation and improvement of hepatic fibrosis, and is expected to be developed into a potential active ingredient of a hepatic fibrosis resisting medicine.

Description

Technical field: [0001] The invention belongs to the field of medicine and relates to the application of D chiral inositol in the preparation of anti-hepatic fibrosis drugs. Background technique: [0002] Liver fibrosis (liver fibrosis) is a pathological process of excessive deposition of diffuse extracellular matrix in the liver. Further progression of liver fibrosis can lead to cirrhosis, liver failure, and portal hypertension, eventually requiring liver transplantation to prolong life. In addition, about 3% of liver cirrhosis patients will transform into liver cancer every year. As one of the malignant tumors that seriously threaten human health, liver cancer is difficult to treat and has a poor prognosis. Rogking, a famous liver disease expert, proposed after in-depth research on liver fibrosis that typical human liver fibrosis can be reversed, but once it develops into middle and advanced liver cirrhosis, it cannot be reversed. This is of great significance for the ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/047A61P1/16
CPCA61K31/047
Inventor 邵荣光李娜仁赵双双金中甲何红伟赵龙吟赵午莉刘虹葛茂旭张镱萱
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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