A microfluidic chip device and method for improving the detection accuracy of resistance pulse method particles
A microfluidic chip and particle detection technology, applied in chemical instruments and methods, laboratory containers, electrochemical variables of materials, etc., can solve the problem of limited chip minimum channel size, undetectable signal, weak detection signal, etc. problem, to achieve the effect of reducing the actual size, improving the signal-to-noise ratio, and optimal detection
Inactive Publication Date: 2017-08-25
DALIAN MARITIME UNIVERSITY
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Problems solved by technology
When using this process, due to the limitation of printing accuracy when printing the mask, the minimum channel size of the processed chip is limited, that is, this processing technology can only process channels with a size larger than 20 microns
When it is necessary to detect small microparticles or nanoparticles, when using this kind of microfluidic chip, due to process limitations, the size of the detection area cannot be further reduced, which will make the detection signal too weak or even undetectable.
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[0030] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings.
[0031] Such as Figure 1-Figure 2 As shown, the microfluidic chip device described in the present invention mainly includes: a DC voltage divider 4, a microfluidic chip 5, a differential amplification element 6, a data acquisition element 7 and a display element 8;
[0032] Such as figure 1 As shown, the microfluidic chip device includes a substrate-PDMS microfluidic chip M with a number of microfluidic channels concavely engraved, and the PDMS microfluidic chip M is concavely engraved with microfluidic channels on one side and the cover sheet- The glass substrate L is sealed into an integrated structure so that a microchannel is formed between the PDMS microfluidic chip and the glass substrate, and the substrate-PDMS microfluidic chip M is made of polydimethylsi...
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The invention discloses a microfluidic chip device and a microfluidic chip method capable of improving the detection precision of resistance-pulse-method particles. The device is provided with a micro-channel, a particle detection region, a first focusing channel and a second focusing channel, wherein the micro-channel comprises a main channel; the main channel is provided with a first main channel liquid storage hole and a second main channel liquid storage hole; the particle detection region is provided with two detection channels, one end of each detection channel is communicated with the main channel and the other ends of the detection channels are connected with two input ends of a differential amplification element respectively; the output end of the differential amplification element is connected with a display element through a data acquisition unit; a first focusing liquid storage hole is formed in one end of the first focusing channel; a second focusing liquid storage hole is formed in one end of the second focusing channel; and the first main channel liquid storage hole, the second main channel liquid storage hole, the first focusing liquid storage hole and the second focusing liquid storage hole are connected with a direct-current voltage splitting device respectively. According to the microfluidic chip device and microfluidic chip method disclosed by the invention, an effective detection width of a detection region is reduced by adopting focusing sheath liquid with high resistivity, so that the detection precision is improved.
Description
technical field [0001] The invention relates to the field of particle detection, in particular to a microfluidic chip device and method capable of effectively improving the particle detection precision of a resistance pulse method. Background technique [0002] Particle detection technology has a wide range of applications in aspects such as environmental monitoring, marine microbial detection, biological small molecule detection, and clinical diagnosis. At present, researchers generally use microscope observation and image processing techniques to detect particles, that is, use microscopes or image sensors to collect particle images, and then convert image signals into digital signals and input them to computers for processing. This type of detection method can detect particle signals, but most of the devices are expensive, take a long time to process, and have limited detection accuracy, which limits their wider application. At the same time, microfluidic chip technology ...
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IPC IPC(8): B01L3/00G01N27/26G01N27/30
CPCB01L3/5027B01L3/502707B01L2300/0809G01N27/26G01N27/30
Inventor 刘志坚宋永欣潘新祥
Owner DALIAN MARITIME UNIVERSITY