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Method for producing selenium-rich grifola frondosa hyphae

A technology of Grifola frondosa and mycelium, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as unstable quality and yield, long cycle of Grifola frondosa, susceptibility to diseases and insect pests, etc., and achieve low Efficient, reasonable and high-value utilization of terminal resources, reduction of production costs, and protection of public health

Active Publication Date: 2016-07-13
SUQIAN ZHONGNONGKE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method of obtaining Grifola frondosa is artificial cultivation, using fruiting body or spore powder as medicine, but artificial cultivation of Grifola frondosa has a long cycle, low production efficiency, high labor intensity, and is subject to seasons and environmental restrictions. and yield instability

Method used

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  • Method for producing selenium-rich grifola frondosa hyphae
  • Method for producing selenium-rich grifola frondosa hyphae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Grifola frondosa strain adopts Grifola frondosa CCTCCM2015065. The amount of rice bran used is 8.0g / 100mL medium, the amount of bran used is 8.0g / 100mL medium, and KH 2 PO 4 0.36g / 100mL, MgSO 4‘ 7H 2 O0.36g / 100mL, cysteine ​​hydrochloride 50mg / 100mL, sodium copper chlorophyllin 10mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use it as a medium for fermentation, shake bottle The sample volume was 40%, the inoculum volume was 10%, and the culture temperature was 28 oC, rotating speed 180r / min, add 2.68mg / 100mL sterilized sodium selenite solution after 2 days of fermentation, the total fermentation time is 7 days. The following steps (3) to (9) in the technical solution described in the foregoing summary of the invention are implemented. The results are: mycelium dry weight 5.8g / 100mL medium, mycelium polysaccharide 266.1mg / 100mL medium, exopolysaccharide 684.2mg / 100mL medium, the content of selenium in mycelium is 52.91μg / g dry...

Embodiment 2

[0072] Grifola frondosa strain CCTCCM2015065 was used. The amount of rice bran used is 5.0g / 100mL medium, the amount of bran used is 5.0g / 100mL medium, and KH 2 PO 4 0.015g / 100mL, MgSO 4‘ 7H 2 O0.015g / 100mL, cysteine ​​hydrochloride 20mg / 100mL, sodium copper chlorophyllin 5mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use as medium for fermentation, shake bottle The sample volume was 40%, the inoculum volume was 8%, and the culture temperature was 24 o C, rotating speed 150r / min, add 0.45mg / 100mL sterilized sodium selenite solution after fermentation 2d, total fermentation time is 5d. The following steps (3) to (9) in the technical solution described in the foregoing summary of the invention are implemented. The results are: mycelium dry weight 3.5g / 100mL medium, mycelium polysaccharide 223.8mg / 100mL medium, exopolysaccharide 312.6mg / 100mL medium, the content of selenium in mycelia is 22.36μg / g dry mycelium , the content of lead is...

Embodiment 3

[0074] Grifola frondosa strain CCTCCM2015065 was used. The amount of rice bran used is 6.4g / 100mL medium, the amount of bran used is 6.4g / 100mL medium, and KH 2 PO 4 0.15g / 100mL, MgSO 4‘ 7H 2 O0.15g / 100mL, cysteine ​​hydrochloride 33mg / 100mL, sodium copper chlorophyllin 7.8mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use as a medium for fermentation, shake The sample volume in the bottle is 40%, the inoculum volume is 9%, and the culture temperature is 26 o C, rotating speed 168r / min, add 1.2mg / 100mL sterilized sodium selenite solution after 2 days of fermentation, the total fermentation time is 7 days. The following steps (3) to (9) in the technical solution described in the foregoing summary of the invention are implemented. The results are: mycelium dry weight 4.1g / 100mL medium, mycelium polysaccharide 219.2mg / 100mL medium, exopolysaccharide 478.5mg / 100mL medium, the content of selenium in mycelium is 39.06μg / g dry mycelium , t...

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PUM

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Abstract

The invention discloses a method for producing selenium-rich grifola frondosa hyphae, and relates to the field of technologies for applying food microorganisms.The method has the advantages that liquid complete rice bran and wheat bran novel cultivation media with added selenium, added cysteine hydrochloride and added sodium copper chlorophyllin are fermented by the aid of novel grifola frondosa strains which are obtained by means of ultraviolet mutagenesis, rice bran and wheat bran are efficiently converted into selenium-rich grifola frondosa hypha raw materials and large quantities of extracellular selenium crude polysaccharides, accordingly, the production cost can be reduced, the yield can be increased, and the selenium-rich grifola frondosa hypha raw materials with high quality can be manufactured; the extracellular selenium crude polysaccharides can be reserved to be further used as deep processing treatment raw materials; the rice bran and the wheat bran which have low value are converted into the selenium-rich grifola frondosa hypha raw materials with diversified healthcare functions, accordingly, beneficial social and economical effects of reducing the production cost, efficient and reasonably utilizing low-end resources and protecting the public health can be realized, and the method is practical.

Description

technical field [0001] The invention relates to the technical field of food microorganism application, in particular to a method for producing selenium-enriched Grifola frondosa mycelium raw material by using a new bacterial strain of Grifola frondosa obtained by mutagenesis. In the method, the new bacterial strain is added with cysteine ​​hydrochloride Salt, sodium copper chlorophyllin and sodium selenite are fermented in a liquid rice bran whole material new medium to obtain the selenium-enriched Grifola frondosa mycelium raw material. Background technique [0002] Medicinal and edible fungi have a long history of use in China and a wide range of users. Modern science reveals that the mycelia, fruiting bodies or spores of commonly used medicinal and edible fungi such as Ganoderma lucidum, Hericium erinaceus, Cordyceps sinensis, and Grifola frondosa can produce amino acids, proteins, vitamins, polysaccharides, nucleosides, Various active ingredients such as flavonoids and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12P19/04C12R1/645
CPCC12N1/02C12N1/14C12P19/04
Inventor 程宇张文丽王冲之刘伟民伍娟朱文静任晓锋王雪梅李婷婷
Owner SUQIAN ZHONGNONGKE BIOTECH CO LTD
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